Chromatic Aberration and What It Could Mean To You. Often we want to image (and measure) the location of different color proteins inside cells. XY registration.

Slides:

Advertisements

Similar presentations

Absorption of photon elevates chromophore to excited state.

Advertisements

Quiz 10 Fall 2011.

< BackNext >PreviewMain Light and Our World Preview Chapter 23 CRCT Preparation.

Microscopy Lecture I.

Microspectrophotometry Validation. Reasons for Changing Instruments Reduced reliability. Limited efficiency. Limited availability and cost of replacement.

RMS VISION SYSTEMS Innovative Solutions for the Semiconductor Industry Application Note: CD Measurement for FET Features NGS 3500 Advanced Metrology System.

Lab 10: Lenses 1.Focal Length 2.Magnification 3.Lens Equation 4.Depth of Field 5.Spherical Aberrations and Curved Focal Plane 6.Chromatic Aberration 7.Astigmatism.

SLAC National Accelerator Center

Chapter 32Light: Reflection and Refraction. Electromagnetic waves can have any wavelength; we have given different names to different parts of the wavelength.

ABRF meeting 09 Light Microscopy Research Group. Why are there no standards? Imaging was largely an ultrastructure tool Digital imaging only common in.

Fluorescence microscopy – Principle and practical consideration Hiro Ohkura.

Ch. 18 Mirrors and Lenses Milbank High School. Sec Mirrors Objectives –Explain how concave, convex, and plane mirrors form images. –Locate images.

© 2005 Pearson Prentice Hall This work is protected by United States copyright laws and is provided solely for the use of instructors in teaching their.

Geometrical Optics and Basic Imaging Light Paths of the Bright Field Microscope E. D. Salmon University of North Carolina at Chapel Hill.

Cameras (Reading: Chapter 1) Goal: understand how images are formed Camera obscura dates from 15 th century Basic abstraction is the pinhole camera Perspective.

Could CKOV1 become RICH? 1. Characteristics of Cherenkov light at low momenta (180 < p < 280 MeV/c) 2. Layout and characterization of the neutron beam.

Optical Center Eugene Hecht, Optics, Addison-Wesley, Reading, MA, 1998.

Fig Spherical aberration for a lens

Could CKOV1 become RICH? 1. Simulations 2. Sensitive area of the detection plane 3. Example of a workable solution 4. Geometrical efficiency of the photon.

Slide 1 Light and telescopes Just by analyzing the light received from a star, astronomers can retrieve information about a star’s 1.Total energy output.

Telescopes. Optical Telescopes Ground based and on satellites Observations are recorded using a camera instead of the human eye most times. – This is.

Integration and Alignment of Optical Subsystem Roy W. Esplin Dave McLain.

Warm Up 1)What does a prism do? a.separates sunlight into ultraviolet and infrared radiation b.separates visible light into several colors c.changes the.

A microscope is a Complicated Lens n The microscope can be represented by a single convex lens.

5 Components Common to All Optical Spectrometers Source Transparent Sample Holder Wavelength Selector Radiation Detector Signal Processor and Readout.

Spherical Aberration Aberration - a departure from the expected or proper course. (Webster's Dictionary) Spherical mirrors have an aberration. There is.

Notes on Chapter 30 Lenses

Lenses Chapter 30.

Spherical Mirrors Spherical mirror – a section of a sphere of radius R and with a center of curvature C R C Mirror.

Dr. Andrew Tomasch 2405 Randall Lab

ASTR-1010 Planetary Astronomy Day Announcements Smartworks Chapters 4: Due Monday, March 1. Smartworks Chapter 5 is also posted Exam 2 will cover.

TIRF Total Internal Reflection Fluorescence Microscopy specialized fluorescence microscopy technique specifically images a very thin optical section (50-250nm)

Fundamental of Optical Engineering Lecture 3.  Aberration happens when the rays do not converge to a point where it should be. We may distinguish the.

Basic Telescope Design Refractors: Utilizes a lens (or lenses) to produce the refraction of light to focus light from an object. The main lens is called.

Evaluating An Image How things look on the screen doesn’t always matter. A full resolution image from a 6 megapixel camera printed on 4” x 6” paper… each.

Copyright © 2010 Pearson Education, Inc. Lecture Outline Chapter 27 Physics, 4 th Edition James S. Walker.

Unit 11: Part 2 Mirrors and Lenses. Outline Plane Mirrors Spherical Mirrors Lenses The Lens Maker’s Equation Lens Aberrations.

3/4/ PHYS 1442 – Section 004 Lecture #18 Monday March 31, 2014 Dr. Andrew Brandt Chapter 23 Optics The Ray Model of Light Reflection; Image Formed.

Optics Real-time Rendering of Physically Based Optical Effects in Theory and Practice Masanori KAKIMOTO Tokyo University of Technology.

Geometric Optics. An object inside the focus casts a virtual image that is only focused by the eye.

Lesson 25 Lenses Eleanor Roosevelt High School Chin-Sung Lin.

1 32 Optical Images image formation reflection & refraction mirror & lens equations Human eye Spherical aberration Chromatic aberration.

T E C H N I C A L B A C K G R O U N D 2. IR CORRECTIVE LENS.

Multispectral Camera Simon Belkin, Audrey Finken, Grant George, & Matthew Walczak Final Project Review Team Parente.

Advanced Fluorescence & Confocal Microscopy 08/2007 Lecture by Dr. Dirk Lang Dept. of Human Biology UCT Medical School Room Phone:

Telescopes. Light Hitting a Telescope Mirror huge mirror near a star * * * small mirror far from 2 stars In the second case (reality), light rays from.

Telescopes Refractors and Reflectors Various Designs Important Properties Modern Advances Beyond Visible Light Refractors and Reflectors Various Designs.

March 31, 2015 Complete Transparent/Translucent/Opaque WS Take out NASA picture (from Friday) Homework: Read & take notes on chapter 23, section 3 (last.

Lenses Properties, Characteristics & Ray Diagrams.

Trivia Question Which “Big Bang Theory” character is an Optical Physicist? (a) Sheldon Cooper (b) Leonard Hofstadter (c) Howard Wolowitz (d) Raj Koothrappali.

Matrix methods, aberrations & optical systems

Applications of Micro Electro Mechanical Systems (MEMS) Jobin Philip.

Use Snell’s law to determine the angle of total internal reflection in the coverslip (without oil). Oil immersion objectives More light (no total internal.

Today’s agenda: Plane Mirrors. You must be able to draw ray diagrams for plane mirrors, and be able to calculate image and object heights, distances, and.

Spherical Aberration. Rays emanating from an object point that are incident on a spherical mirror or lens at different distances from the optical axis,

2011 년 11 월 4 일 (11 일 수업 보강 ) 제 9 장.  Light Waves  Mirrors: Plane and Not so Simple  Refraction  Lenses and Images  The Human Eyes  Dispersion and.

Telescopes I. Refraction: Refraction is the _____________ of light as it passes through glass. II. Reflection: Reflection occurs when light _____________.

Date of download: 7/8/2016 Copyright © 2016 SPIE. All rights reserved. Through-the-objective TIRF creates the evanescent field on the aqueous side of the.

Optical Non-Invasive Approaches to Diagnosis of Skin Diseases

Telescopes and the Electromagnetic Spectrum Section 3

Lab 10: Lenses Focal Length Magnification Lens Equation Depth of Field

Optical Non-Invasive Approaches to Diagnosis of Skin Diseases

Brooke Young, Makenna Cooper, Javier Aranguren

MFM setup. MFM setup. The excitation lasers are combined in a fiber through an acousto-optic tunable filter, collimated, reflected on a dichroic mirror.

Investigation 22.3: Images from Lenses Name: ________________________

Refraction Optical Phenomena.

Refraction Optical Phenomena.

Reader/Task focuser Cutter 1 Folder Taper Colorist/Cutter

L2036H1 grid6 First cut x x x x x x x x x x x x x x x x x x x x x x x

Presentation transcript:

Chromatic Aberration and What It Could Mean To You. Often we want to image (and measure) the location of different color proteins inside cells. XY registration is easy, but it is not so simple in Z. Chromatic Aberration and What It Could Mean To You. Michael Cammer Dec. 2011

Different of light focus at different Z axis positions

150X Olympus objective well corrected Emissions from 500 to 670 nm. 4umbeads_150X_Z020_XZ

Example with transwell assay

How to calibrate This is caused by chromatic aberration. The reflection is at 488 nm and the multiphoton excitation is at 840 nm. Based on measurements with beads, the focal point from one to the other wavelength shifts by approximately 3 um.

Chromatic aberration a problem in TIRF too

Detailed instructions at dex.html

Conclusion When doing precise colocalization, need to check the offset of different wavelength probes along the optical axis. When taking single optical section pictures of different probes, need to adjust focal planes appropriately.