Chapter 16

In General…… What is the definition of volume and what is the basic unit? How do you use a graduate cylinder to measure volume? What are the instruments used to measure volume? In order of increasing accuracy, list the instruments that are used to measure volume. How are micropipettors validated?

Glassware –Calibrated either TC or TD-what does this mean? –What are the tolerances for a volumetric in comparison to a graduated cylinder? –Compare and contrast the use of volumetrics, graduated cylinders, beakers and flasks –What is a serological pipet? –What is a Mohr pipet? –What is a Pasteur pipet?

Micropipetting Devices Positive displacement vs air displacement

Pipetting of Difficult Solutions Air displacement micropipettors are not very accurate when dispensing volatile liquids (like acetone) or viscous materials (like protein solutions). Review Table 16.3

Validation of Micropipettors Box 5 pages Problems –All