Che 440/540 2D-NMR

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Presentation transcript:

Che 440/540 2D-NMR

Relaxation delay 90 o pulse  t 1 (microseconds) 90 o pulse Prototype Pulse Sequence for a 2D-NMR Experiment Acquire (t 2 seconds)

Stacked plot of 22 spectra of acetone Projection of (a) Resulting interferogram Origin of the Second Dimension

Fourier transform of a series of interferograms creates the second dimension.

A Simulated 1 H- 1 H Correlated Spectroscopy (COSY) Spectrum

Actual COSY Spectrum of Isobutanol A, B A B B,C C

COSY Spectrum of Ipsenol

Basics of HETCOR and HMQC Heteronuclear correlated (HETCOR) and heteronuclear multiple quantum coherence (HMQC) spectroscopy show direct correlations between hydrogens and their directly bonded carbons. In HETCOR the signal is detected by observing 13 C, and in HMQC by observing 1 H. The HMQC experiment is shorter, but the pulse sequence is more complicated. Both give the same information: one-bond 1 H- 13 C correlations

13 C chemical shifts 1 H chemical shifts no correlation A simulated HMQC spectrum

HMQC Spectrum of Ipsenol diastereotopic CH 2 ’s

COSY Spectrum of Strychnine