ClearH 2 O Duration Assessment of DietGel 31M & HydroGel Bilsky Laboratory University of New England August 6, 2012

Protocol 4 week old male C57BL/6J mice (Jackson Laboratory) were received into the animal facility on June 28 th, 2012 Mice were marked, weighed, and randomly housed 4 per cage into the three treatment groups: Control (n=12) – Standard food and water DietGel 31M (n=12) – DietGel 31M and standard water HydroGel (n=12) – Standard food and HydroGel Mouse were kept in the vivarium under standard housing conditions (temperature 22 ± 2°C and relative humidity between 55-60%) Bodyweights were measured once a day to the nearest 0.05 gram at 8 AM for 31 days On days 14 and 21 mice were inspected by blinded laboratory personnel for overall health (e.g. coat and typical behavior) On day 21 mice were visually inspected for tooth length

Data shows the average bodyweight of mice on DietGel 31M versus control for 14 days and the average bodyweight of mice on HydroGel versus control for 14 days A 2 way ANOVA revealed no statistical significance between the three treatments through the 14 days DATA: 14 Days Researcher: Alex Colville and Denise Giuvelis

Data shows the average bodyweight of both DietGel 31M and HydroGel versus control treatments for the entire 31 day study *, **, *** denotes significance after a 2-way ANOVA with p < 0.05, 0.01, and respectively DATA: 31 Days Researcher: Alex Colville and Denise Giuvelis Dates: 6/28-7/28/12

Conclusions: General Health Blinded observation revealed no difference in appearance of the coat of the mice or any changes in behavior due to the varying treatment groups Due to the hard rodent chow diet being replaced by a soft gel diet, tooth length was monitored Tooth length was observed on day 21 and it was determined by a blinded observer that there were no differences between treatment groups