Monitoring Equipment Used in the Microbiology Laboratory 1.

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Presentation transcript:

Monitoring Equipment Used in the Microbiology Laboratory 1

2 Equipment - Microbiology Monitor temperatures of: – incubators – refrigerators – freezers Perform function checks for: –pipettes –autoclaves –pH meter –weights and measures Maintain records

3

4 Sterilization Sterile media reagents materials Safe disposable waste Preparing media reagents materials Contaminated waste

5 Two Stage Sterilization Controls Process control Chemical indicators Bacteriological indicators Sterile products control Growth test

6 Sterilization indicators

7 Sterility Indicators Chemical indicators Temperature for sterilization have been met. Do not address time or pressure Biological indicators Temperature, time and pressure for sterilization have been met NOTE: Neither indicator is a guarantee of sterilization if: Autoclave is overpacked Broth volumes are too large

8 Sterilization Logbook

9 Microscopes Keep lens clean Have at least one oil immersion lens (X100) Use spring loaded lens Keep non-immersion lens free of oil Do not allow sample to migrate above the lens Maintain Kohler Illumination