Novel Mouse Model of Monocular Amaurosis Fugax by Dominique Claude Lelong, Ivan Bieche, Elodie Perez, Karine Bigot, Julia Leemput, Ingrid Laurendeau, Michel Vidaud, Jean-Philippe Jais, Maurice Menasche, and Marc Abitbol Stroke Volume 38(12):3237-3244 December 1, 2007 Copyright © American Heart Association

Figure I. Branches of the internal carotid artery in relation to the ventral surface of the cranium in the rat (eye vascularization has not been determined accurately by other teams in the mouse).12. Figure I. Branches of the internal carotid artery in relation to the ventral surface of the cranium in the rat (eye vascularization has not been determined accurately by other teams in the mouse).12 Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association

Figure 1. Fluorescence microscopy of flat-mounted, fluorescein-perfused retinas (group A). Figure 1. Fluorescence microscopy of flat-mounted, fluorescein-perfused retinas (group A). Right ischemic retinas were compared with left control retinas from the same animal. The surgical procedure interrupted eye vascularization. Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association

Figure 2. Fluorescence microscopy of flat-mounted, fluorescein-perfused right retinas at 2 reperfusion times (5 minutes and 1 hour) after acute ischemia for 15 minutes (group B15), 30 minutes (group B30), and 60 minutes (group B60). Figure 2. Fluorescence microscopy of flat-mounted, fluorescein-perfused right retinas at 2 reperfusion times (5 minutes and 1 hour) after acute ischemia for 15 minutes (group B15), 30 minutes (group B30), and 60 minutes (group B60). The surgical procedure was reversible. Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association

Figure 3. A, Summary of results (mean±SEM) showing the variation in a-wave amplitudes recorded under scotopic conditions with different flash intensities 4 weeks after 30 minutes of ischemia (n=11) or sham operation (n=11). Figure 3. A, Summary of results (mean±SEM) showing the variation in a-wave amplitudes recorded under scotopic conditions with different flash intensities 4 weeks after 30 minutes of ischemia (n=11) or sham operation (n=11). A significant decrease (P<0.05*) in the a-wave amplitude was observed for flash intensities of 1, 3, 10, and 25 cds/m2 (respectively, P=0.039, P=0.013, P=0.005, P=0.007). B, Summary of results (mean±SEM) showing the variation in b-wave amplitudes recorded under scotopic conditions with different flash intensities 4 weeks after 30 minutes of ischemia (n=11) or sham operation (n=11). A decrease in b-wave amplitude was significantly different (P<0.05*) for flash intensities of 0.1, 0.3, 1, 3, 10, and 25 cds/m2 (P=0.038, P=0.013, P=0.005, P=0.009, P=0.004, P=0.012, respectively). Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association

Figure 4. A, Summary of results (mean±SEM) showing the variation in a-wave amplitudes recorded under photopic conditions with different flash intensities 4 weeks after 30 minutes of ischemia (n=11) or sham operation (n=11). Figure 4. A, Summary of results (mean±SEM) showing the variation in a-wave amplitudes recorded under photopic conditions with different flash intensities 4 weeks after 30 minutes of ischemia (n=11) or sham operation (n=11). B, Summary of results (mean±SEM) showing the variation in b-wave amplitudes recorded under photopic conditions with increasing flash intensities 4 weeks after 30 minutes of ischemia (n=11) or sham operation (n=11). A decrease in b-wave amplitude was significantly different (P<0.05*) for flash intensities of 10 and 25 cds/m2 (P=0.046, P=0.003, respectively). Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association

Figure 5. Measurements of retinal layers thicknesses 4 weeks after 30 minutes of retinal ischemia or sham operation. Figure 5. Measurements of retinal layers thicknesses 4 weeks after 30 minutes of retinal ischemia or sham operation. A, Peripheral retina. A slightly but nonsignificantly decreased total retina thickness was observed between the ischemic group (n=13) and the sham group (n=12, mean±SEM). This possible decreased retinal thickness suggests the potential existence of minor histologic lesions in the peripheral regions of the ischemic retina. B, Central retina. No difference in retinal layer thicknesses (mean±SEM) was observed between the ischemic (n=13) and sham (n=12) groups. TR indicates total retina; PE, pigmentary epithelium; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; and IPL, inner plexiform layer. Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association

Figure II. Comparison of sham-operated and ischemic mouse retinas stained with hematoxylin and eosin 4 weeks after 30-minute ischemia. Figure II. Comparison of sham-operated and ischemic mouse retinas stained with hematoxylin and eosin 4 weeks after 30-minute ischemia. Typical light photomicrographs of paraffin-embedded transverse sections of the peripheral retina (A) and central retina (C) from sham-operated eyes and the peripheral retina (B) as well as the central retina (D) from ischemic eyes. GG indicates ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; RPE, retinal pigmented epithelium; CB, ciliary body; and ON, optic nerve. Lelong D et al. Stroke 2007;38:3237-3244 Copyright © American Heart Association