Clinical Trial Results. org The ERASE Trial: Effect of rHDL on Atherosclerosis-Safety and Efficacy Presented at American College of Cardiology Annual Scientific Session 2007 Presented by Dr. Jean-Claude Tardif, M.D. ERASE Trial

Clinical Trial Results. org ERASE Trial: Background CSL-111 is an apolipoprotein A-I isolated from human plasma and phosphatidylcholine derived from soybean.CSL-111 is an apolipoprotein A-I isolated from human plasma and phosphatidylcholine derived from soybean. Commercial development of the cholesteryl ester transfer protein inhibitor torcetrapib was recently stopped after a trial showed an increase in mortality with torcetrapib.Commercial development of the cholesteryl ester transfer protein inhibitor torcetrapib was recently stopped after a trial showed an increase in mortality with torcetrapib. CSL-111 is an apolipoprotein A-I isolated from human plasma and phosphatidylcholine derived from soybean.CSL-111 is an apolipoprotein A-I isolated from human plasma and phosphatidylcholine derived from soybean. Commercial development of the cholesteryl ester transfer protein inhibitor torcetrapib was recently stopped after a trial showed an increase in mortality with torcetrapib.Commercial development of the cholesteryl ester transfer protein inhibitor torcetrapib was recently stopped after a trial showed an increase in mortality with torcetrapib. ACC 2007

Clinical Trial Results. org ERASE Trial: Background CSL-111 is a different class of agents targeting increasing HDL and is not a CETP inhibitor but is derived from human plasma.CSL-111 is a different class of agents targeting increasing HDL and is not a CETP inhibitor but is derived from human plasma. The goal of this trial was to evaluate the effect of plaque burden of reconstituted HDL compared with placebo among patients with recent acute coronary syndromes.The goal of this trial was to evaluate the effect of plaque burden of reconstituted HDL compared with placebo among patients with recent acute coronary syndromes. CSL-111 is a different class of agents targeting increasing HDL and is not a CETP inhibitor but is derived from human plasma.CSL-111 is a different class of agents targeting increasing HDL and is not a CETP inhibitor but is derived from human plasma. The goal of this trial was to evaluate the effect of plaque burden of reconstituted HDL compared with placebo among patients with recent acute coronary syndromes.The goal of this trial was to evaluate the effect of plaque burden of reconstituted HDL compared with placebo among patients with recent acute coronary syndromes. ACC 2007

Clinical Trial Results. org CSL mg/kg n=111 ERASE Trial: Study Design CSL mg/kg n=12 Placebo Matching Dose n= patients aged years with clinical need for coronary angiography, defined as ≥ 1 narrowing ≥20% coronary angiography at baseline; within 2 weeks of having an acute coronary syndrome defined as unstable angina or non-ST-segment or ST-segment elevation MI Placebo-controlled. Randomized. Double-blinded. Mean follow-up: 6 weeks. 17% female. 183 patients aged years with clinical need for coronary angiography, defined as ≥ 1 narrowing ≥20% coronary angiography at baseline; within 2 weeks of having an acute coronary syndrome defined as unstable angina or non-ST-segment or ST-segment elevation MI Placebo-controlled. Randomized. Double-blinded. Mean follow-up: 6 weeks. 17% female. ACC 2007  Primary Endpoint: Percentage change in atheroma volume from baseline to 6 weeks  Secondary Endpoint: Absolute change in plaque volume, change in plaque characterization indexes on IVUS, and change in coronary score on quantitative coronary angiography.  Primary Endpoint: Percentage change in atheroma volume from baseline to 6 weeks  Secondary Endpoint: Absolute change in plaque volume, change in plaque characterization indexes on IVUS, and change in coronary score on quantitative coronary angiography. 80 mg group discontinued 6 week follow-up 80 mg group discontinued 6 week follow-up R

Clinical Trial Results. org ERASE Trial: Primary Endpoint Percent Change in Atheroma Volume from Baseline to 6 weeks The primary endpoint of percent change in atheroma volume from baseline to 6 weeks did not differ between treatment groups (-3.4% in the CSL-111 group vs. -1.6% in the placebo group, p=0.48)The primary endpoint of percent change in atheroma volume from baseline to 6 weeks did not differ between treatment groups (-3.4% in the CSL-111 group vs. -1.6% in the placebo group, p=0.48) PlaceboCSL-111 Change in atheroma volume (%) ACC 2007 p = 0.48

Clinical Trial Results. org ERASE Trial: Secondary Endpoint Absolute Change in Atheroma Volume from Basleine to 6 weeks The absolute change in atheroma volume from baseline to 6 weeks also did not differ between treatment groups (-5.3mm 3 in the CSL- 111 group vs mm 3 in the placebo group, p=0.39)The absolute change in atheroma volume from baseline to 6 weeks also did not differ between treatment groups (-5.3mm 3 in the CSL- 111 group vs mm 3 in the placebo group, p=0.39) PlaceboCSL-111 Absolute change in atheroma volume (mm 3 ) Absolute change in atheroma volume (mm 3 ) ACC 2007 p = 0.39

Clinical Trial Results. org ERASE Trial: Secondary Endpoints Mean change in plaque characterization index on IVUS was for CSL-111 and for placebo (p=0.01).Mean change in plaque characterization index on IVUS was for CSL-111 and for placebo (p=0.01). Reduction in coronary score on angiography was significantly less with CSL-111 compared with placebo ( mm for CSL-111 vs mm for placebo, p=0.03).Reduction in coronary score on angiography was significantly less with CSL-111 compared with placebo ( mm for CSL-111 vs mm for placebo, p=0.03). Mean change in plaque characterization index on IVUS was for CSL-111 and for placebo (p=0.01).Mean change in plaque characterization index on IVUS was for CSL-111 and for placebo (p=0.01). Reduction in coronary score on angiography was significantly less with CSL-111 compared with placebo ( mm for CSL-111 vs mm for placebo, p=0.03).Reduction in coronary score on angiography was significantly less with CSL-111 compared with placebo ( mm for CSL-111 vs mm for placebo, p=0.03). ACC 2007

Clinical Trial Results. org ERASE Trial: Limitations The 80 mg CSL-111 group was discontinued due to high rates of abnormal liver function tests, including alanine aminotransferase >5 times the upper limit of normal (ULN) in 50% of the group and aspartate aminotransferase >5 times ULN in 33% of the group.The 80 mg CSL-111 group was discontinued due to high rates of abnormal liver function tests, including alanine aminotransferase >5 times the upper limit of normal (ULN) in 50% of the group and aspartate aminotransferase >5 times ULN in 33% of the group. The lipid effects of the agent in the study were not reported.The lipid effects of the agent in the study were not reported. A larger trial would be required to evaluate the clinical safety and efficacy of this agent.A larger trial would be required to evaluate the clinical safety and efficacy of this agent. The 80 mg CSL-111 group was discontinued due to high rates of abnormal liver function tests, including alanine aminotransferase >5 times the upper limit of normal (ULN) in 50% of the group and aspartate aminotransferase >5 times ULN in 33% of the group.The 80 mg CSL-111 group was discontinued due to high rates of abnormal liver function tests, including alanine aminotransferase >5 times the upper limit of normal (ULN) in 50% of the group and aspartate aminotransferase >5 times ULN in 33% of the group. The lipid effects of the agent in the study were not reported.The lipid effects of the agent in the study were not reported. A larger trial would be required to evaluate the clinical safety and efficacy of this agent.A larger trial would be required to evaluate the clinical safety and efficacy of this agent. ACC 2007

Clinical Trial Results. org ERASE Trial: Summary Among patients with recent acute coronary syndromes, treatment with the novel reconstituted HDL agent CSL-111 was not associated with a reduction in atheroma volume compared with placebo.Among patients with recent acute coronary syndromes, treatment with the novel reconstituted HDL agent CSL-111 was not associated with a reduction in atheroma volume compared with placebo. Treatment with CSL-111 was associated with a change in atheroma volume within the treatment arm from baseline to 6 week follow-up.Treatment with CSL-111 was associated with a change in atheroma volume within the treatment arm from baseline to 6 week follow-up. Among patients with recent acute coronary syndromes, treatment with the novel reconstituted HDL agent CSL-111 was not associated with a reduction in atheroma volume compared with placebo.Among patients with recent acute coronary syndromes, treatment with the novel reconstituted HDL agent CSL-111 was not associated with a reduction in atheroma volume compared with placebo. Treatment with CSL-111 was associated with a change in atheroma volume within the treatment arm from baseline to 6 week follow-up.Treatment with CSL-111 was associated with a change in atheroma volume within the treatment arm from baseline to 6 week follow-up. ACC 2007