AGRAZINC 100 www.agranco.com

WHAT IS AGRAZINC 100 AGRAZINC 100 is a concentrated for of micro-encapsulated ZINC OXIDE For a more effective release int the gastrointestinal tract of animals. For treatment against DIARREAH.

MODE OF ACTION The lipid coating in AGRAZINC 100 is disolved by hy lipase enzyme when it reached the duodenum and ZINC OXIDE is slow released into the area for immediate action. The micro-encapsulation prevents the AGRAZINC 100 from being adosorbed or changed before it gets to the duodenum. The micro-encapsulation PREVENTS the natural negative effect on palatibility by ZINC OXIDE.

AGRAZINC 100: Effects on post weaned piglets 1 Design 90 hds at avg. 24 days, 3 hds per pen(30 pens) Place and Period: Florida (US), for 28 days (OCT, 2006)

AGRAZINC 100: Effects on post weaned piglets 1 Diet design(normal) 1-12 days : CP 22%, Total lysine 1.6% 13-28 days : CP 18%, Total lysine 1.25% * all diets are included with 100ppm ZnSO4 Treatments Control: normal diet+ZnO, 150ppm Zn Treated 1(T1): normal diet+ ZnO, 3000ppm Zn Treated 2(T2): normal diet+ AGRAZINC 100, 200g/T

AGRAZINC 100: Effects on post weaned piglets 1 0.462 0.457 0.345 P<0.05 [United States, 2006]

AGRAZINC 100: Effects on post weaned piglets 1 0.7 0.694 0.578 P<0.05 [United States, 2006]

AGRAZINC 100: Effects on post weaned piglets 1 0.67 0.65 0.6 P<0.05 [United States, 2006]

AGRAZINC 100: Effects on post weaned piglets 1 13.4 % 3.7% 3.2% P<0.05 [United States, 2006]

AGRAZINC 100: Effects on post weaned piglets 2 Design Post weaned piglets 90 hds at avg. 20 days, 5 hds/pen(18pens), 3 replications Place and Period: Florida (US), 28 days (DEC, 2006) Diets design (normal) and materials 1-8 days: 1.5% total lysine 9-28 days: 1.3% total lysine ZnO: Zn 72% Antibacterial agent: Carbadox 55mg/kg

AGRAZINC 100: Effects on post weaned piglets 2 Treatments control: normal diet Treated 1(T1): control+ AGZ 200g/T T2: control+ ZnO 3000ppm T3: control+ Carbadox 55mg/kg T4: T3+ AGZ 200g/T T5: T4+ ZnO 3000ppm

AGRAZINC 100: Effects on post weaned piglets 2 Daily Gain, g P<0.05 Carbadox 55mg/kg, No Carbadox [United States, 2006]

AGRAZINC 100: Effects on post weaned piglets 2 Daily Feed Intake, g P<0.05 Carbadox 55mg/kg, No Carbadox [United States, 2006]

AGRAZINC 100: Effects on post weaned piglets 2 AGRAZINC 100 give ‘synergy effect’ to producers when it was used with AGPs AGRAZINC 100 200g/t can replace the effect of ZnO at 3000 ppm, effectively.

AGRAZINC 100: Effects on post weaned piglets 3 Place and Period National Swine Reaserch and Training Center(Thailand), 35 days Design Post weaned piglets 32 hds at 35 days, 8 hds/pen(4pens) Infected 10 ml of 107cfu of E. coli and 10 ml of 107 cfu of haemolytic E. coli(K88) in every pig in the 3th days after weaning and keep the data same parameter Antibiotics(Colistin 100ppm)

AGRAZINC 100: Effects on post weaned piglets 3

AGRAZINC 100: Effects on post weaned piglets 3

AGRAZINC 100: Effects on post weaned piglets 3 AGRAZINC 100 treatment had better performance, even in case of haemolytic E. coli(K88) infection. AGRAZINC 100 show ‘synergy effects’ when it was used with antibiotics. AGRAZINC 100 is the effective alternative for pharmacological level of ZnO in animal diets

AGRAZINC 100: Effects of ZnO sources on piglet’s performance(2007 AGRAZINC 100: Effects of ZnO sources on piglet’s performance(2007. April) Trial design and Place 6 weeks old piglets weaned at 35 days, 60 heads. 20 heads/group, 3 test groups. 24 days trail Dr. Wang, Ji-Hui(Prof.), Dalian Polytechnic Univ.(China) Trial diets design Control: normal diet spec. + ZnO 3000ppm T1: normal diet spec. + Agrazinc 300ppm T2: normal diet spec. +Agrazinc 100ppm * Effects of pharmaceutical dosage of ZnO is well known, so we have conducted this trial without ‘negative control without 3000ppm’ intentionally. Diet : Corn-soy based diet

Measuring Items Feed Intakes Average Daily Gain(ADG) FCR Diarrhea rate(%) Metabolism value, % pH profile in digestive tract Microorganism profile in digestive tract

Avg. Daily Feed Intake(ADFI) 0.897±0.162 0.816±0.333 0.730±0.074 ADFI, kg

Avg. Daily Gain(ADG) 0.483±0.050 0.459±0.109 0.318±0.166 ADG, kg

FCR 2.753±0.468 1.978±0.169 1.512±0.022

Diarrhea rate (%) 3.45±3.28 2.11±2.40 Diarrhea rate, % 1.48±1.92

Dry Matter(DM) Metabolism value, % 88.09±0.86 86.90±1.44 DM metabolism , % 81.95±1.76

Metabolism value, % Metabolism value, % 85.42 83.01 76.34 74.39 65.23 64.02 61.77 50.19 48.53 Metabolism value, %

pH profile in digestive tract 7.70 7.29 7.11 7.08 7.06 6.88 6.28 6.19 pH value 6.17 6.15 6.18 6.07

Microorganism profile in digestive tract: E. coli, log10 7.14 6.73 6.40 6.26 6.20 5.66 5.56 5.52 5.42 5.44 5.28 E. coli, log10 4.74

Microorganism profile in digestive tract: Lactobacillus acid., log10 8.89 8.88 8.81 7.82 7.69 8.66 7.76 7.66 7.35 7.20 Lactobacillus acid., log10 6.51 6.14

Bifidobacterium bif. , log10 Microorganism profile in digestive tract: Bifidobacterium bifidum, log10 9.80 9.62 9.49 9.39 9.40 9.24 9.23 9.07 8.12 8.03 Bifidobacterium bif. , log10 7.59 7.10

Summary AGRAZINC 100… It showed superior beneficial effects in growth, digestion, diarrhea and microorganism in the gut of piglet in comparison with normal pharmaceutical dosage of ZnO. It means we can reduce inclusion level of ZnO in animal diets with sustainable performances.. ..and avoid environmental pollution. In addition, AGRAZINC 100 also showed a good performance in micro organism control in the gut; reduction in E. coli. It may replace AGPs in diets successfully.

AGRAZINC 100: benefits Treat and prevent Diarrhea Minimize the excretion of Zn: prevent environmental pollution Better Feed Intake Alternative for AGPs ADG & FCR improvement Reduce pH in the gut Increase the activity of beneficial micro organism Reduction in pathogen; E. coli etc.

AGRAZINC 100 INCLUSION RATES: PIGLETS and CALVES: 200 gr/MT of feed (if they encounter severe case, 300 gr/MT are recommened) SWINES,LAYERS AND BROILERS: 100 gr / MT of feed DAIRY AND BEEF CATTLE: 4 GRAMS/HEAD PER DAY HORSES: 2 GRAMS / HEAD PER DAY SHEEP: 2 GRAMS PER HEAD PER DAY.