Class switch recombination Immunglobulin variable Region (Antigenbindung) konstante Region (Eigenschaften, Funktion)

Slides:



Advertisements
Similar presentations
Specific Defenses of the Host: The Immune Response Immunogen: A substance that induces a specific immune response Antigen (Ag): A substance that reacts.
Advertisements

TODAY B CELL DEVELOPMENT.
How is antibody diversity generated? Two early theories: Germline hypothesis The genome contains many loci encoding antibody molecules. B cells express.
Immunoglobulin Gene Rearrangement MCB720 January 20, 2011 Presented by: Alamzeb Khan & Maria Muccioli
Antibody Diversity.
Immunoglobulin Gene Rearrangement
Generation of diversity in lymphocyte antigen receptors Jan. 31, Feb. 2 & 5 Chapter 4.
Outline Immunoglobulin Superfamily Antigen Recognition Members:
Antibodies and T Cell Receptor Genetics 2011
Chapter 14 B Lymphocytes. Contents  B cell receptor and B cell complex  B cell accessory molecules  B cell subpopulations  Functions of B cells 
P ART I Immnoglobulins are proteins Proteins are specified by genes There are too few genes to specify all the antibodies. –i. e., ~32,000 genes < 10,000,000,000.
Organization & Expression of Immunoglobulin Genes
Diversification of antibodies after B-cells encounter antigen Alternative splicing Somatic hypermutation Ig. class switch.
Control of gene expression Transcriptional Post-transcriptional Epigenetics and long range control.
Immunology 6 Specificity 8. Specificity of immunoglobulin molecule on B cell – BCR of receptor on T cell – TCR is defined and produced before their exposition.
Organization and Expression of Immunoglobulin Genes.
B-CELL DIFFERENTIATION IN THE PERIPHERY SOMATIC HYPERMUTATION
Chapter 4 and 5 Ig study questions (Th): How does the immune system recognize a diverse universe of possible antigens? How do antibodies simultaneously.
B Cell Activation and Antibody Production Lecture 15.
V(D)J Recombination and the Role of CTCF Richard Wolfe; Xinhao Liu.
Aims Gene rearrangement and class switching of B-cell Igs.
Antibodies & Antigens1 Antibodies Also chap 5 pp Self-Test Questions: Chap 4: all Chap 5: D all MolnQuiry.
Epigenetic control of Gene Regulation Epigenetic vs genetic inheritance  Genetic inheritance due to differences in DNA sequence  Epigenetic inheritance.
The genetic basis of antibody structure
Clonal Selection. Antibody Structure Made up of 4 polypeptide chains Made up of 4 polypeptide chains –2 identical heavy chains –2 identical light chains.
Chapter 4 and 5 Ig study questions (Tu): Can you name at least four ways in which CSR and V(D)J recombination differ? What are the substrates (what genes,
Supplemental Figure 1 Piceatannol SP , ER Inhibitors concentration (µM) A B.
Ig Polypeptides Are Encoded by Multiple Gene Segments LIGHT CHAIN
Rearrangement The normal process by which antibodies and T cell receptors are made.
Farshid Yeganeh PhD. Virtually any substance can be the target of an antibody response. "Beadle and Tatum in 1941 One gene-one polypeptide hypothesis“
Chapter 13 Lymphocyte Maturation and Antigen Receptor Expression
ADAPTIVE IMMUNITY. Adaptive immunity Specific Slow during the primary response, but very fast during the secondary responses memory.
A a Activated B-cell Mature naive B-cell Memory B-cell B-CELL DIFFERENTIATION IN THE PERIPHERY SOMATIC HYPERMUTATION ISOTYPE SWITCH Ag.
Arbelos Area of Arbelos. What’s the area of the shaded (grey) region?
IgGs: Somatic recombination and combinatorial diversity n Immune system - recognition of “self” vs. “non-self” n Hallmarks of immune response –specificity.
Chapter 5 Organization and Expression of Immunoglobulin Genes Dr. Capers.
Lymphocyte Development and Antigen Receptor Gene Rearrangement Chapter 8.
B Cell Activation Abul K. Abbas UCSF FOCiS.
Immunoglobulin Gene Rearrangement
A a Activated B-cell Mature naive B-cell Memory B-cell B-CELL DIFFERENTIATION IN THE PERIPHERY SOMATIC HYPERMUTATION ISOTYPE SWITCH Ag.
Adaptive Immunity Central objective: Protect against foreign invaders memoryCreate memory of invasion to prevent recurrent infection specificResponse.
Antibody Diversity. Immunoglobulin: antibody Antibody response: B cells, with the help of T cells, produce antibody to antigen, preserve the ability to.
Antigens and Antibody Structure
Immunoglobulin — Structure and Function
Immunoglobulin Gene Rearrangement
The genetic Basis of Ab Structure
Immunology Overview Kristine Krafts, M.D..
Unit-1 Introduction to Java
Control of gene expression
Transformation Chapter 12.
Immunoglobulins structure and classification
ADAPTIVE IMMUNE RESPONSE
Immunity and Blood Groups
Control structures Chapter 3.
Immunoglobulin Expression and the Role of Antigen in
Antibody production and B cell differentiation
Immunoglobulins (2 of 2) Ali Al Khader, MD Faculty of Medicine
Locus control regions by Qiliang Li, Kenneth R. Peterson, Xiangdong Fang, and George Stamatoyannopoulos Blood Volume 100(9): November 1, 2002.
3. Lymphocytes Journal of Allergy and Clinical Immunology
Fifty years later: Emerging functions of IgE antibodies in host defense, immune regulation, and allergic diseases  Hans C. Oettgen, MD, PhD  Journal of.
Genetics in the Generation of Antibody Diversity
Analysis of clonotypic switch junctions reveals multiple myeloma originates from a single class switch event with ongoing mutation in the isotype-switched.
Biochemistry & Functions Dr Sara Mariyum
._,.,._.,,,.., ,.,,,, _,....., ­ µ. qt......
Immunoglobulins (2 of 2) Ali Al Khader, MD Faculty of Medicine
Abbas Chapter 8 Lymphocyte Development and the
Somatic Hypermutation of Immunoglobulin Genes
Molecular mechanisms of IgE regulation
Volume 15, Issue 5, Pages (March 2005)
Generation of antibody diversity
Presentation transcript:

Class switch recombination Immunglobulin variable Region (Antigenbindung) konstante Region (Eigenschaften, Funktion)

Class switch recombination DNA Ig heavy chain 53 EµIµ PSµCµCδCδIγ PSγSγCγCγIε PSεSεCεCεIα PSαSαCαCα3RR variabel konstant Eµ = Enhancer IP = Promotor für jeweilige konstante Region S = switch Region, repetitive Sequenz C = konstante Region Regionen µ, δ, γ3, γ1, γ2b, γ2a, ε, α 3RR= regulatorische Region downstream der C, 4 Enhancer VDJ

Class switch recombination DNA Ig heavy chain 53 EµIµ PSµCµCδCδIγ PSγSγCγCγIε PSεSεCεCεIα PSαSαCαCα3RR 5 SµSαSαCαCα 3 loop VDJ Promotor aktiviert

Class switch recombination DNA Ig heavy chain 53 EµIµ PSµCµCδCδIγ PSγSγCγCγIε PSεSεCεCεIα PSαSαCαCα3RR 5 SµSαSαCαCα 3 funktionsfähiges switch- Produkt mit Deletion hier: IgA switch circle VDJ