Template provided by: “posters4research.com” Diagnostic markers for Soil-borne wheat mosaic virus resistance in soft red winter wheat Yuanfeng Hao 1, Yingying Wang 1, Zhenbang Chen 1, Dan Bland 1, Gina Brown-Guedira 2, Jerry Johnson 1 * 1 Department of Crop and Soil Sciences, University of Georgia, Griffin Campus, GA 30223, USA 2 USDA-ARS Plant Science Research, Department of Crop Science, North Carolina State University, Raleigh, NC 27695, USA * Corresponding author: Soil-borne wheat mosaic virus Acknowledgement Abstract We recently showed that two co-dominant SSR markers Xbarc161 and Xbarc177 were closely linked with the major QTL for SBWMV resistance in Pioneer 26R61 and AGS The two markers were suitable for marker- assisted selection in Pioneer 26R61 background. The aim of this study was to validate the marker effectiveness in genetically diverse backgrounds. Seventy-five advanced lines in 2010 Gulf Atlantic Wheat Nursery (GAWN), and 38 tested lines in 2011 Uniform Eastern Soft Red Winter Wheat Nursery (UESRWWN) were used. These lines were mainly contributed by the public breeding units from 15 States in USA, covering nearly all winter wheat growing regions, and other few lines were provided by private company including Limagrain, Syngenta/AgriPro, Trio Research Inc. and Pioneer Hi- Bred. Pioneer 26R61 and AGS 2000 were used as positive and negative checks for marker detection, respectively. For the 2010 GAWN material, the phenotype data was collected in Plains, GA, and genotype was conducted in Small Grains Lab in University of Georgia. Out of 75 lines, 73 showed the consistency between the phenotype and the genotype, and two other lines were negative for linked markers but resistant to SBWMV in the field. Therefore, new gene (s) might be present in the two lines. And for the 2011 UESRWWN material, the genotype data for the 38 lines totally agreed with the phenotype data in Clarksville, MD. The genotype work was conducted by USDA-Eastern Regional Small Grains Genotyping Lab in North Carolina State University. These results demonstrate that Xbarc161 and Xbarc177 are highly diagnostic for detection of SBWMV resistance in diverse genetic material, and also can be used as a starting point for discovering new resistant genes for SBWMV in wheat. The authors acknowledge the financial support by the National Research Initiative of USDA’s Cooperative State Research, Education and Extension Service, CAP (Grant No ). Contact Linked markers of QSbm.uga-5DL 2010 GAWN * Yuanfeng Hao UGA, Griffin Campus 1109 Experiment St., Griffin QSbm.uga-5DL was detected across all environments based on the linkage maps of chromosome 5D in the Pioneer 26R61/AGS 2000 population (a) and the AGS 2020/LA population (b). The QTL region is indicated as a red rectangle  Soil-borne wheat mosaic virus (SBWMV) is an important viral disease in US, and can practically destroy the entire crop of a susceptible cultivar.  SBWMV belongs to genus Furovirus, vectored by the plasmodiophorid Polymyxa graminis, can survive in soil for decades until a suitable host plant is encountered.  Utilization of resistant varieties is currently the only practical and environmentally friendly means for controlling SBWMV.  Development of resistant cultivars is restrained by germplasm screening in the field, however, quick and accurate laboratory-based marker-assisted selection can facilitate the selection procedure.  Two co-dominant SSR markers Xbarc161 and Xbarc177, closely linked with SBWMV resistance at Sbm1 locus on chromosome 5DL, were previously identified (Hao et al TAG, submission). Cultivar/LineXbarc161Xbarc177SbmField reaction LA02006E SCW010025G LA04089D-P USG GA E GA LE VA08W SCW010025H SCW010025K SCTX98-27-J AR AR VA08W VA08W NC SCLA99049D-E1-J VA08W-286-+?5.0 NC AR NC SCW010025T LA01069D SS8641 CK AR NC AR LA01139D VA06W GA EE GA E VA08W AR AR AR B AR LA VA08W AGS GA EE LA01034D-42-3-C SCTX98-20-J SCW010025G LA01059D VA08W NC GA E NC VA08W AR NC SCW010025D NC FL03169D LA02150E VA07W LA02007E GA E LA01145D C VA08W GA E SCW010025L LA02015E NC NC GA E GA LE VA08W NC AR GA LE GA E AR SCLA01111C-J NC SCTX98-27-J Cultivar/LineXbarc161Xbarc177SbmField reaction INW Branson+++0 Bess+++0 Shirley+++0 T MO OH IL IL TN NC X08-18A3+++0 X08-39D+++0 G G G IL OH OH T T GA LE GA LE MH ±±±0 MH P04702A P0537A P06403A KY02C KY02C VA05W VA08W VA08W TN NC MSU Line E MSU Line E MSU Line E UESRWWN * * GAWN: Gulf Atlantic Wheat Nursery * UESRWWN: Uniform Eastern Soft Red Winter Wheat Nursery The distribution of the tested lines in GAWN & UESRWWN in 15 States of the USA, the number means the amount of lines The typical amplification of marker Xbarc177 in GAWN entry G1-G38, P26R61 is positive check, and AGS 2000 is negative