COMPARISON OF BIOAVAILABILITY OF DIFFERENT FORMS OF CURCUMIN USING NON-EVERTED RAT INTESTINAL SAC MODEL

Turmeric is dried yellow powder obtained from the rhizomes of Curcuma longa Family Zingiberaceae Curcumin is the most plentiful of the curcuminoids found in turmeric Biocurcumax™ is a patented extract of the rhizome of Curcuma longa, standardised to provide 95 % of natural mixed curcuminoids and sesquiterpenoids Curcumin is consumed as turmeric in many forms either as a spice in our daily meals or as a traditional remedy Turmeric Rhizomes Curcuma longa Plant INTRODUCTION

1, 7-bis (4-hydroxy-3-methoxyphenyl) -1, 6-heptadiene-3, 5- dione Molecular formula C 21 H 20 O 6 Molar mass g/mol Curcumin is, yellow pigment, active component of turmeric It is orange-yellow crystalline powder, insoluble in water Also called diferuloyl methane

Physicochemical properties – Practically insoluble in water – Log P : 2.05 – Undergoes hydrolytic degradation at alkaline pH Unstable in presence of light Curcumin is unstable in the gut and the traces that pass through the GI tract rapidly degrades or is conjugated through glucuronidation Ingestion of 2 to 10 grams of curcumin resulted in undetectable to very low serum levels Limited clinical application due to poor aqueous solubility, poor absorption and extensive metabolism leading to decreased bioavailability

Traditional Vehicles Fats And milkWaterMilkGhee TRADITIONAL VEHICLES FOR INGESTION OF CURCUMIN

USES OF CURCUMIN PHARMACOLOGICAL PROPERTIES TRADITIONAL USES As spices and colorant As cosmetic As Blood purifier As a remedy for ulcer Common cold, wound, Sore throat, leprosy inflammation etc. Anticancer activity Antioxidant activity Antidiabetic activity Antirheumatic activity Antimicrobial activity Hepatoprotective and Nephroprotective activity

AIMS AND OBJECTIVE C OMPARISON OF BIOAVAILABILITY OF DIFFERENT FORMS OF CURCUMIN USING NON - EVERTED RAT INTESTINAL SAC MODEL BIOCURCUMAX PURE CURCUMIN TURMERIC ?

EXPERIMENTAL Male wistar rats (weighing g) were used for the study The clean intestinal tract was prepared into 8 ± 0.2 cm long sacs having a diameter of 3 ± 0.5 mm Each sac was filled with 0.5 ml of drug suspension and tied with thread to a length of approximately 5 cm Each non-everted intestinal sac was placed in a glass conical flask containing KRPBS (50 ml) with provision for aeration The sacs were maintained at 37 °C in a shaking water bath operating at 50 strokes per min Samples were withdrawn from outside the sacs every 30 min for 8 h and replaced with fresh buffer The samples were analyzed spectrophotometrically at λ max 422nm

RESULTS AND DISCUSSION

Graphs showing % Permeation in 8 h from different vehicles

Results of the study indicate that: - % Permeation in 8 h from the rat intestine was maximum for Corn oil from all the forms of curcumin - The pattern of % Permeation profile for different forms of curcumin from Corn oil is: Biocurcumax(26.3) > Turmeric(19.9)> Pure curcumin(13.4) - The pattern for % Permeation profile from different vehicles is: Corn oil(26.3)> R.Butter(16.9)> Milk(6.8)> Aq. Suspension(2.5) COMPARITIVE PERMEATION PROFILE OF DIFFERENT FORMS OF CURCUMIN FROM DIFFERENT VEHICLES

The Present study concludes that: - % Permeation of curcumin increase as the lipophilicity of the vehicle increases - Permeation is maximum from Biocurcumax followed by Turmeric and Pure curcumin - Curcumin should be consumed with oily vehicles instead of water as Biocurcumax or Turmeric for its maximum therapeutic effects REFERENCES: 1. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV, Curcumin: from ancient medicine to current clinical trials, Cell. Mol. Life Sci., 2008, 65 (11): 1631–52 2. Ruan LP, Chen S, Yu BY, Zhu DN, Cordell GA, Qiu SX, Prediction of human absorption of natural compounds by the non-everted rat intestinal sac model, Eur. J. Med. Chem., 2006, 41: REFERENCES: 1. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV, Curcumin: from ancient medicine to current clinical trials, Cell. Mol. Life Sci., 2008, 65 (11): 1631–52 2. Ruan LP, Chen S, Yu BY, Zhu DN, Cordell GA, Qiu SX, Prediction of human absorption of natural compounds by the non-everted rat intestinal sac model, Eur. J. Med. Chem., 2006, 41: curcumin turmeric CONCLUSIONS