Judi Tilghman, Ph.D.Shelly Staat Product Manager Genomics and Proteomics Integrated Systems 2001 International Sales Meeting 2001 MultiPROBE II Product Focus

Packard BioScience Increase visibility of MPII platforms in the genomics & proteomics market Genomics Developing co-marketing agreements with reagent vendors  Validated protocols on MPII, HT & Talon platforms –Development work being done in-house, at vendor sites & customer sites  Application notes with joint customers  Provide customer references  Provide easy access to protocols, app notes and posters from our website via reciprocal web links

Packard BioScience Millipore Montage Plasmid Miniprep Montage PCR Cleanup vacuum filtration proprietary size exclusion filter plates

Packard BioScience RNAture Isolation of mRNA 96-Well Format  Automation friendly  High-throughput capability Fast Process Easy Protocol  3 steps standard protocol  One-step high- throughput protocol mRNA Express™ Kit

Packard BioScience Prolynx Utilizes proprietary super- paramagnetic particles (patent pending) Particles bind dye terminators, not DNA extension products, “reverse purification” No centrifuge rotors or carriers, or vacuum station Particles are pre-loaded in a standard 96-well and 384-well formats RapXtract Dye Terminator Removal Kit

Packard BioScience Macherey-Nagel silica membrane technology in combination with vacuum processing fully automated, high-throughput purification of plasmid DNA & total RNA high-throughput clean-up of PCR products NucleoSpin® Robot Plasmid kit NucleoSpin ® Multi - 96 Extract NucleoSpin ® Multi - 96 RNA NucleoSpin® Robot Plasmid kit NucleoSpin ® Multi - 96 Extract NucleoSpin ® Multi - 96 RNA

Packard BioScience Genomics - Summary  Qiagen –Validated protocols on MPII, HT, Talon platforms Qiaprep plasmid prep –Development of PCR cleanup protocol in progress –Application note in progress  Millipore –Validated protocols on MPII, HT platforms for Montage & MultiScreen plasmid prep & pcr cleanup Protocols on Talon platform in progress 2 application notes in progress

Packard BioScience Genomics - Summary RNAture –mRNA express kit currently being developed in- house Prolynx –RapXtract kit currently being developed in-house Macherey-Nagel –Validated protocol for Nucleospin 96 plasmid prep kit –Application note in progress Future collaborations with: –Promega, Sigma and Chemagen

Packard BioScience Proteomics The study of proteins expressed by genomes Completion of the sequencing of the 1st draft of human genome indicates there are approximately 250,000 proteins in the human genome Only 2-5% of proteins in human genome have been identified Genome (DNA) Transcriptome (mRNA) Proteome (protein)

Packard BioScience Increase visibility of MPII platforms in the genomics & proteomics market Proteomics  Developing strategic marketing relationships with key players in the proteomics market –Micromass –Applied Biosystems –Bruker –Millipore  OEM agreements  Promotion of MPII platforms as “stand alone” system for Proteomics sample prep & spotting of MALDI targets

Packard BioScience SamplePrep2-D GELS OR BLOTS MASS SPEC MALDI-TOFSpotCutterProteinDigestMALDISpotting Sample SWISS-PROT/ TrEMBL ESIMS/MS Imaging D/BASE PepSeq The proteomic process SWISS PROT/ TrEMBL MultiPROBE MicroMass ABI Bruker MultiPROBE

Packard BioScience Digestion Protocol De-stain 100mM ammonium bicarbonate andacetonitrile Dehydrate Acetonitrile Reduce 10mMdithiothreitol in 100mM ammonium bicarbonate, 30 minutes Alkylate 55mMiodoacetamide in 100mM ammonium bicarbonate, 20 minutes Wash 100mM ammonium bicarbonate Dehydrate Acetonitrile Digest 6ng/µL Trypsin in 50mM ammonium bicarbonate (25 µL), 5 hours at 37ºC Extract 1% formic acid / 2%acetonitrile (30 µL) Spot 96-spot MALDI target (2 x 2.5 µL)

Packard BioScience The proteomic process ProteinDigestMALDISpotting MASS SPEC MALDI-TOF Micromass ABI Bruker MALDI Targets Range From 96 Grid on 2 X2 Format to 384 Grid on Standard Microplate Format MultiPROBE

Packard BioScience Proteomics

Packard BioScience Proteomics

Packard BioScience Proteomics Millipore ZipTips® P10 size disposable tip which contains varying µg amounts of C18 Solid Phase Tips are used to de-salt and concentrate extracted proteins prior to spotting on to a MALDI target plate ZipTips with collars are currently available from Packard Millipore will take over distribution within next 6 months ZipTips will be packaged in “robotic friendly” Packard tip box

Packard BioScience Proteomics - Summary  Micromass –OEM with MPII standard Expand to include HT Forecast units in 2001  Applied Biosystems –Strategic Marketing agreement in progress MPII, HT and Talon platforms  Millipore –ZipTip compatibility –Developing automated platform for “vacuum based in-gel digest kit”

Packard BioScience Digestion Protocol De-stain 100mM ammonium bicarbonate andacetonitrile Dehydrate Acetonitrile Reduce 10mMdithiothreitol in 100mM ammonium bicarbonate, 30 minutes Alkylate 55mMiodoacetamide in 100mM ammonium bicarbonate, 20 minutes Wash 100mM ammonium bicarbonate Dehydrate Acetonitrile Digest 6ng/µL Trypsin in 50mM ammonium bicarbonate (25 µL), 5 hours at 37ºC Extract 1% formic acid / 2%acetonitrile (30 µL) Spot 96-spot MALDI target (2 x 2.5 µL)

Packard BioScience What does this mean for you? More visibility in the proteomics market = more MPII sales as stand alone systems