Staph and Strep.

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Presentation transcript:

Staph and Strep

Cocci Morphology

Catalase Test Enzyme Detoxify hydrogen peroxide H2O2  H2O + O2 (+) bubbles Staphylococcus (-) no bubbles Streptococcus Enterococcus Detoxify hydrogen peroxide H2O2  H2O + O2

Columbia - CNA Selective for GP Differential Colistin Nalidixic Acid Blood hemolysis

BAP: Hemolysis Alpha Gamma

BAP Hemolysis: Beta

Gram Stain: Staph

MSA Selective Differential 7.5 % NaCl, halotolerant Staphylococci pH indicator: phenol red Mannitol Fermentation Acid end products Yellow Non fermentation = red

MSA

DNase Differential Dye Enzyme Results Methyl green pH 7.5 hydrolytic Clear zone = (+)

Mueller-Hinton Agar (MH) AB sensitivity Novobiocin Disc Sensitive > 18 mm Resistant < 18 mm Staph chart for ID

Staph Tri-plates Mannitol Growth NF DNAse (+) Novobiocin (S)

Staph Tri-Plates Mannitol Growth Fermentation DNAse (+) Novobiocin (S)

Staph Tri-plate Mannitol Growth NF DNAse (-) Novobiocin (R)

Staph Tri-plate Mannitol Growth Fermentation DNAse (-) Novobiocin (R)

Coagulase Test Staph aureus Converts fibrinogen to fibrin Immune defense Protection from phagocytosis

Rapid Staph Test Staph aureus Virulence Factors Results Coagulase (+) Fibrinogen  Fibrin Clots Protein A Fc regions of IgG Inhibits Ab Results Color Change Clumping

Anaerobic Systems Facultative Anaerobes Staph Capnophiles Strep

Streptococcus GP cocci Chains Catalase (-) Capnophiles Groups Lancefield Groups Surface Antigens

Lancefield Strep Classifications Group A S. pyogenes Group B S agalactia Group C S. equi Group D Enterococcus faecalis S. bovis Group K S. salivarius Viridans S. mutans S. mitis Pneumoocci S. pneumoniae

Catalase and MSA Catalase (-) MSA No growth Except Not halotolerant Enterococcus (-)

Hemolysis Types

Bacitracin and SXT Sensitivities

Zone of Inhibition

Ab Sensitivities Bacitracin SXT Any zone = Sensitive No clearing = Resistant SXT Zone > 20 mm = S Zone < 20 mm = R

Bile Esculin Selective Differential Results Bile salts Enteric organism growth Differential Hydrolyze esculin (CH2O) Reacts with FeNH3-citrate Results Growth Black precipitate

CAMP Identify Group B Strep (GBS) CAMP factor enhances hemolysis Arrow Head produced for GBS

Rapid Strep Test Group A Strep Immunoassay Results Ag ID Ab on test strip Results Color change

Flow Chart