Cellular response to DNA damage Reponse Mechanisms Tolerance of DNA damage Replicative bypass of template damage Translesion DNA synthesis Reversal.

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DNA damage and repair summary

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Presentation transcript:

Cellular response to DNA damage Reponse Mechanisms Tolerance of DNA damage Replicative bypass of template damage Translesion DNA synthesis Reversal of DNA damage Enzymatic photoreactivation Repair of O 6 -alkylguanine, O 4 - alkyltimidine Ligation of DNA strand breaks Excision of DNA damage Base Excision Repair Nucleotide Excision Repair Mismatch Repair Double strand breaks ligation Single strand annealing Recombinational Repair Non-Homologous End Joining

La radiazione Solare può essere distinta in 3 componenti principali a seconda della lunghezza donda: ULTRAVIOLETTO ( nanometri) 10% VISIBILE ( nm.) 45% INFRAROSSO ( nm.) 45%

UV-induced damage

UV-responsive photolyases

Direct reversal (de-alkylating proteins)

Base Excision Repair

Nucleotide Excision Repair (E.coli)

Nucleotide Excision Repair (Global Genome Repair -Humans)

Nucleotide Excision Repair (Transcription Coupled -Humans)

Common features of GGR & TCR