Lecture 05 – Extracellular Signal Receptors III Lecture 05 – Extracellular Signal Receptors III BIOL 5190/6190 Cellular & Molecular Singal Transduction Prepared by Bob Locy Last modified -13F
Extracellular Signal Receptors II 1.Voltage-gated ion channels (VOCs) 2.Ligand-gated ion channels
Ion channel receptors Voltage gating
Ion channel receptors Voltage Operated Channel Multisubunit – 1, 2, , , & Calcium channel – in PM – contains voltage sensor 1 – also contains drug binding sites Large cytosolic loop, links to synaptic vessicle and/or ryanodine receptors 2 – extracellular, regulatory, glycosylation – transmembrane region – intracellular and functions to mediate intracellular effects in different cell types
Ion channel receptors Detailed structure 1 has 4 repeat domains (I-IV) each with 6 MSDs MSD6 of each domain makes the pore has 4 alpha helices, but no MSDs 2 and (single MSD) associate via disulfide has 4 MSDs Regulation by protein kinase A (AKAP18) phosphorylation.
Ion channel receptors 1.2 L-type channel (shown) differs in regulation from 1.1 L-type channel PKC Calumodulin (CAM) G-protein regulation of PP2A
Ion channel receptors
Ion channel receptors – ionontrophic glutamate receptors
Ion channel receptors Cyclic nucleotide- gated channels
Ion channel receptors Calcium signaling and intracellular calcium channels IP3 Ryanodine
Ion channel receptors IP3 Receptor animation
Ion channel receptors Ryanodine
Ion channel receptors