The Need for Food Animal Survival Standard Grade Biology

Learning Outcome 1 Explain in simple terms why food is required by animals.

Learning Outcome 2 State the chemical elements present in carbohydrates, fats and proteins Describe the simple structure of carbohydrates, proteins and fats in terms of simple sugars, amino acids, fatty acids and glycerol.

You are what you eat!! In this lesson you will –Look at what is a balanced diet –Carry out food tests on Starch Glucose Protein Fat –Discuss what happens if you don’t eat a balanced diet

Balanced Diet

What is a balanced diet ? Your diet must include five groups of food substances –Carbohydrates, fats, proteins, minerals and vitamins It must also include water and fibre. A balanced diet is a diet that provides enough of these substances in the correct proportions to keep you healthy.

Carbohydrates Carbohydrates give us energy. Chemical elements – C, H and O. Starch is made from simple sugars.

Testing for glucose 1.Pour some glucose solution into a test tube 2.Add a few drops of benedict's solution 3.Heat in a water bath

Testing For Starch Put a few drops of starch solution on a spotting tile. Add a few drops of iodine.

Proteins Proteins are needed for growth and repair. Proteins are made from amino acids Chemical elements – C, H, O, N (and S)

Testing for Proteins 1.Put 2 ml of protein solution in a test tube 2.Add 2ml of Biurets reagent

Fats Fats are an energy store, protecting vital organs and providing insulation. Fats contain C, H and O Fats are made from a glycerol molecule and three fatty acids.

Testing for fat (the emulsion test) Add a few drops of cooking oil into a test tube Add 2cm 3 ethanol and shake Add 2cm 3 water and shake again.

What happens if you don’t eat a balanced diet. What is this cartoon trying to demonstrate? Can you think of any examples of health problems that arise from not eating a balances diet? Give three examples and explain the effects on health.

Learning Outcomes 3 State that digestion is the breakdown of large particles of food into smaller particles to allow absorption into the blood stream through the small intestine wall. Explain that digestion involves the breakdown of insoluble food substances into soluble food substances

What happens to the food we eat?

The gut as a production line

Learning Outcome 4 identify in a diagram / model the main parts of the mammalian alimentary canal and associated organs (mouth, salivary glands, oesophagus, stomach, pancreas, liver, gall bladder, small intestine, large intestine, appendix, rectum and anus) state the sites of production of the main digestive juices in mammals (salivary glands, stomach, pancreas, liver, small intestine)

Digestion Digestion is the breakdown of large, insoluble food molecules into small, soluble food molecules so that they can be absorbed into the blood stream.

Pupil Activity Label the diagram of the digestive system. –Use the textbook supplied to help if needed –Design a table to link the structure and function of different parts of the digestive system.

Salivary glands stomach trachea tongue mouth anus rectum colon pancreas Large intestine appendix Small intestine ileum Duodenum Gall bladder liver oesophagus

Mammalian teeth The Need for food Animal Survival SG Biology

Learning Outcomes (5) To know the different structure and functions of each type of tooth To understand the adaptations of a herbivore, omnivore and a carnivore to feeding.

Feeding in mammals Mammals have teeth which may be used to bite pieces of food and to chew it into smaller pieces before swallowing. The shapes of teeth are suited to their function. –Incisors and canines are used for biting –Premolars and molars are used for chewing and crushing food.

Patterns of Teeth

Human Jaw

teeth There are different types of teeth, each specially shaped to perform a particular job. –Incisor - A broad flat sharp tooth found at the front of the mouth. Designed for biting and cutting food. –Canine - A sharp pointed tooth for piercing flesh and tearing. –Pre-molar & molar - A broad flat tooth with many cusps. Its rough surface is used for crushing, grinding and chewing food

Carnivore adaptations Look at the dog skull, and the diagrams of a dogs skull. With the aid of a diagram – make notes explaining how the dogs teeth and jaws are adapted for a carnivorous diet.

Herbivore adaptations Look at the sheep’s skull, and the diagrams of a sheep skull. With the aid of a diagram – make notes explaining how the sheep's teeth and jaws are adapted for a herbivorous diet.

Learning Outcome 6 Explain the mechanism of peristalsis Explain how the contractions of the stomach help in the chemical breakdown of food.

Digestion of Food The digestion of food can either be mechanical or chemical. Mechanical digestion includes –Chewing –Action of muscles in oesophagus, stomach and small intestine In chemical digestion enzymes catalyse the breakdown of larger food molecules into smaller food molecules.

Digestion in the mouth and oesophagus Chewed food is mixed with saliva in the mouth Saliva contains –Amylase is an enzyme which starts to digest starch into sugars (maltose) –Mucus helps soften the food making it easier to swallow The bolus travels down the oesophagus with the aid of peristalsis

Peristalsis in the gullet

Learning Outcome 7 State that different enzymes are responsible for the breakdown of carbohydrates, proteins and fats. Give an example of an amylase, a protease and a lipase. State their substrates and products.

Digestion of Starch Take 2ml of 1% starch solution –Test for starch –Test for sugars Take 2 ml 1% starch solution, add 2ml 2% amylase solution –Leave for 20 minutes –Test for starch –Test for sugar

Action of amylase on starch Put two drops of iodine solution into all the wells on a white spotting tile Add 2ml 1% starch solution and 2ml 2% amylase solution to a test tube. Every minute, test the contents of the test tube for starch. Write a conclusion on your results

Protein Digestion Protein digestion occurs in the stomach and small intestine. Protease enzymes are produced by the gastric pits in the stomach lining or by the pancreas. In the stomach, hydrochloric acid is also produced which provides the optimum pH for trypsin to work (as well as killing bacteria in food)

Protein Digestion Add 2ml of protein solution to three test tubes. Number the test tubes 1, 2 and 3 1.Add 1ml HCl 2.Add 1ml Water 3.Add 1ml HCl Do not add the trypsin until last 1.Add 0.5 ml water 2.Add 0.5 ml trypsin 3.Add 0.5ml trypsin

Protein digestion Leave for 10 minutes Test each of the test tubes for the presence of protein using Biurets reagent. Explain your results.

Fat Digestion Milk does not dissolve in water, in the digestive system, bile produce by the liver (stored in the gall bladder) emulsifies fats to give a larger surface area for enzymes to work on. You are going to use phenolphthalein to investigate the effect of bile on the digestion of fat.

Fat Digestion Take 4 test tubes and number them 1, 2, 3 and 4. set up the test tubes as shown in the table below. Test tube1234 Milk 3 ml Bile 00.5ml1 ml Water 1ml0.5ml00

Fat Digestion Add 5 drops of phenolphthalein to each test tube Add sodium carbonate to each test tube until the solution goes pink. Add 0.5ml of lipase to test tubes 1, 2 and 3. Add 0.5 ml water to test tubes 4. Observe your results. Try to explain what you are observing.

Chemical Digestion Enzymes –Are proteins –Are produced by cells –Change chemical substances into new products –Are “specific” to one substance –Work best at their “optimum temperature” (around 30 – 40 o ) –Work best at an optimum pH

Digestive enzymes There are different types of digestive enzyme –Proteases break down proteins into amino acids –Lipases break down fats into fatty acids and glycerol –Amylase breaks down starch (carbohydrase enzyme) into maltose (sugar) Maltose is then broken down by maltase to form glucose

Chemical Digestion There are also other substances in the digestive system which help with the breakdown of food. Hydrochloric acid –Secreted by stomach –Kills the bacteria in food –Enzymes in stomach work best at a low pH e.g. pepsin

Chemical Digestion Hydrogen carbonate ions –Secreted by pancreas –Neutralises acid, enabling enzymes in small intestine to work Bile –Produced by liver, stored in gall bladder, secreted into small intestine –Emulsifies fats (Larger surface area for enzymes to work on)

Learning Outcome 8 State that only small soluble molecules can pass through the wall of the small intestine. Explain how the structure of the small intestine is related to its function. Explain how the structure of a villus, including the lacteal and the blood capillaries, are related to the absorption and transport of food. Describe the role of the large intestine in water absorption and elimination.

Digestion in the small intestine 3 important liquids are added to the food –Pancreatic juice (carbohydrases, proteases and lipases) –Bile (neutralises acid and emulsifies fat) –Intestinal juice (carbohydrases, proteases and lipases)

What happens next?

Absorption in the ileum The small intestine is well designed for absorption, it has –Thin lining –A good blood supply –A very large surface area (about 9m 2 )

Absorption in the Villi

The Model Gut A model of absorption

“the model gut”

Making a model gut Wash a 12cm length of visking tubing (A) in warm water. Tie a knot in one end Fill the tubing with 10cm3 of starch and glucose solution(B). Wash the outside of the tubing. Put it into a boiling tube containing DI water (C) (holding it in place with a rubber band. After 20 minutes, test the surrounding water for starch and glucose. CBACBA

Comparing the model gut with the villi Draw conclusions from the experiment, explaining what the results were, and how the model gut represents the real situation E.g. what does the water represent, what does the Visking tubing represent, etc.

Large intestine and the elimination of waste. A watery mix of enzymes and undigested food (mainly fibre) moves into the colon. Water is absorbed back into the blood stream. Faeces are compacted in the rectum and egested through the anus.

The end What do they think they are looking at?

Learning Outcome 9 To revise the learning outcomes taught in this sub topic.

What happens to the food we eat?

The gut as a production line