Tensile testing Structural Properties –Load –Deformation –Stiffness –Ultimate load –Ultimate elongation –These properties are dependent on cross- sectional.

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Tensile testing Structural Properties –Load –Deformation –Stiffness –Ultimate load –Ultimate elongation –These properties are dependent on cross- sectional area and length of specimen Mechanical Properties –Strain energy density –Modulus –Ultimate strain –These properties are independent of CSA and length (i.e. same no matter what geometry is tested

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization

Freeze clamps Also called cyrojaws. Use liquid N2 to freeze Must maintain freeze line

VDA Video extensometer Local strains

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization

Strain rates Avulsion versus mid-substance- –Some theorize that these effects are due to strain rate, but REALLY depends on Age.

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization

Mature versus immature, but then look at mature versus OLD

Age Puberty-adult see an increase in ult. Load Open epiphysis-avulsion Closed epiphysis (skeletally mature)- midsubstance Old-usually avulsion Stiffness decreases with age Ultimate load decreases with age –-see plot

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization

Temperature Increase temp-decrease stiffness Race horses Tennis shoes

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization

Waterbath

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization

How do we properly store a specimen for transplantation? –Freezing effects?? –Irradiation?? Disease transmission

Pre-tension on graft

Influences on testing Gripping (freeze clamps) Strain Rate Age Temperature Hydration Immobilization Exercise