23 and 25 October, 2006 Chapter 16 Regulation in Prokaryotes
Overview Transcriptional initiation is the most common point to regulate gene expression. Any of the events of initiation, including polymerase binding and open complex formation may be regulated either positively or negatively. Regulation is accomplished by sequence-specific DNA binding proteins. Binding may be promoter proximal or at a distance. DNA footprinting and mobility shift assays are used to investigate the binding of regulatory proteins. In the E. coli lac operon, there are both repressors and activators, each of which is allosterically regulated. Many regulatory systems control a large number of genes and operons, like the catabolite repression and heat shock regulons. NtrC is regulated by covalent modification, binds at a distance, and hydrolyzes ATP to pronmote open complex formation. MerR activates transcription by twisting the promoter. Riboswitches regulate transcription or translation without protein mediators. Phage lambda uses alternative regulatory systems to control lytic or lysogenic growth. Repressor and Cro compete to determine lytic or lysogenic growth, in response to the stability of the CII protein. Downstream regulation in lambda involves antitermination.
Activators and repressors may regulate binding of polymerase.
Some activators regulate open complex formation.
Cooperative Binding and Transcriptional Regulation at a Distance
The lac operon
lac operon regulation
Control Regions and lac Operator Half-sites
RNA polymerase can form open complexes even in the presence of the LacI protein.
RNA polymerase interacts with promoter and CAP
Helix-turn-helix Interactions with DNA
CAP bends DNA
Activator Bypass
Lac repressor binds as a tetramer
Genetic experiments with partial diploids elucidated the ideas behind regulation of gene expression.
Regulation by Alternative -Factors
Regulation of GlnA by -54 and NtrC.
NtrC Acts at a Distance
MerR Regulation
AraC Regulation
Arabinose relaxes loops, and the loops reform in the absence of competitor.
Regulation of the trp operon
Tryptophan Interaction with Trp Repressor
Attenuation
Ribosomal proteins regulate their own translation.
Riboswitches regulate gene expression without regulatory proteins.
Phage lambda
Lambda Genome
Lambda Control Region
Lambda Repressor and Binding Sites
Cooperative Binding
Cooperative and Non-Cooperative Binding
Lambda Regulation
Negative Autoregulation
DNA Looping Between two lac operators
CII Control of the Lytic / Lysogenic Decision
N and Q Antiterminators
int Regulation
Title