Metagenomics Rob Edwards MCS. The Soudan Mine, Minnesota Red Stuff Oxidized Black Stuff Reduced.

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Presentation transcript:

Metagenomics Rob Edwards MCS

The Soudan Mine, Minnesota Red Stuff Oxidized Black Stuff Reduced

Red and Black Samples Are Different Cloned and 454 sequenced 16S are indistinguishable Black stuff Red Cloned Red

There are different amounts of substrates in each environment Black Stuff Red Stuff

There are different amounts of metabolism in each environment

Nitrification differentiates the samples Edwards (2006)

The SEED metagenomics server

Metabolic potential in environments

Phylogenomics

Comparing Metagenomes to Genomes (or other metagenomes!)

Lots of sequences all pyrosequencing

Metabolic potential in environments

Sulfur CDA 60.2% CDA 21.7% Respiration Capsule Motility Membrane transport Stress Signaling Phosphorus RNA Mine Saltern Marine Microbialites Coral Fish Animals Freshwater From sequences to environments Dinsdale et al. unpublished