Scanning em of C. elegans. Crawl movie Fly-in movie.

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THE WORM Caenorhabditis elegans as a model organism.
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Presentation transcript:

Scanning em of C. elegans

Crawl movie

Fly-in movie

Male, scanning em

Male tail scanning em

Rene Mating Movie

Embryonic cell lineage

D lineage

ABala lineage

AIML neuron AIML lineage

AIML circuit

Z1Z2 Z3 Z4 Postembryonic blast cells

Adult hermaphrodite anatomy

C. elegans typical cross section

Seam cell postembryonic lineages in the male wild type lin-4(e912) reiterations

wild type lin-14(sd) repeats S1 lin-14(lf) skips S1

lin-4(e912) lin-14(sd) lin-4(lf) and lin-14(gf) have the same phenotype: they repeat S1.

LIN-4S is complementary to sequences in the 3’UTR of lin14.

Human miRNA genes

Symmetric neuron phenotype of lsy-6(-)

A regulatory pathway leading to neuron asymmetry involving two micro RNA’s

Worms Eating Bacteria Expressing dsRNA to a GFP Reporter Gene Activate RNAi wild type worms RNAi-defective mutants GFP lost in embryos GFP lost in the gut from Mello and Conte, Nature 431,

RNAi in Plants 1928 report of development of viral (tobacco ringspot virus) resistance in tobacco, now known to be due to endogenous RNAi report of development of viral (tobacco ringspot virus) resistance in tobacco, now known to be due to endogenous RNAi.

RNAi/miRNA Pathways from Meister and Tuschl, Nature 431, , 2004.

The Structure of Argonaute (Slicer) Song et al., Science 305, , 2004.