Lecture 21: Molecular Tools of Genetic Diagnosis Reading Assignment: Chapter 42, pgs. 488-504; Harper’s Biochemistry (25 th edition). Objective: To understand.

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Lecture 21: Molecular Tools of Genetic Diagnosis Reading Assignment: Chapter 42, pgs ; Harper’s Biochemistry (25 th edition). Objective: To understand the theory and application of molecular biology to the diagnosis of disease. I. Definition of molecular biology II. Enzymes used in molecular biology A. Restriction endoculeases B. DNA polymerases C. DNA ligases III. Techniques for detection of gene mutations A. Polymerase Chain Reaction B. Allele Specific Oligonucleotide C. Restriction Fragment Length Polymorphism

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