Circadian Susceptibility to Pyrethroids in Female Drosophila melanogaster By David Chin Mentor: Dr. Jadwiga Giebultowicz
Relevance for Research How fruit flies metabolize toxins may be: Different between males and females Related to how humans metabolize toxins via circadian rhythms These links may open up new inquiries in pharmaceuticals, horticulture, and global economics
Circadian Rhythms Internal clocks that control when organisms rest and remain active I.e. Jet lag… … … Controlled by the Period and Tim genes
Circadian Clocks (cont.)
Circadian Rhythms (cont.) Physiologically, they influence: Hormone secretion Oxygen consumption Metabolism Other functions of the body
Point of Interest Circadian rhythms may influence the rate of metabolism of many substances, including toxins The pyrethroid, deltamethrin, is administered to the female flies to investigate how they metabolize toxins in relation to the presence and absence of the circadian rhythm
Pyrethroids Synthetic insecticides derived from the flowers of pyrethrums or several plants from the genus Chrysanthemum mons/f/fa/Chrysanthemums.jpg
Deltamethrin Deltamethrin is a pyrethroid insecticide that damages the nervous system, paralyzing the organisms after cuticular penetration or oral uptake of the neurotoxin C 22 H 19 Br 2 NO 3
Deltamethrin (cont.) Modifies the membrane function proteins and keeps the voltage-dependent sodium channels continuously open
Flies Being Tested CS Flies = Wild type flies with Period & Tim genes Per-null Flies = Mutant flies that lack the Period gene expressions Csp Flies = Backcrossed flies that express the Period & Tim genes and are closely related to the Per-null flies
Experiments Dose Response Two Hour Interval Test GST Assay Glutathione S-Transferases take part in metabolizing toxins
Mass Ratio Data Average mass of female = mg Average mass of male = mg
Dose Response Data
Two Hour Interval Test Data
Csp and Per-Null Testing Data
GST Assay Data
Future Investigations I will continue working on investigating direct toxin applications and GST assays throughout the school year at Oregon State University
Acknowledgements Howard Hughes Medical Institute URISC Dr. Kevin Ahern Dr. Jadwiga Giebultowicz Everyone in the lab