JM - 1 Systems biology of cell-signaling systems: It's all about protein-protein interactions Jarek Meller Departments of Environmental.

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Presentation transcript:

JM Systems biology of cell-signaling systems: It's all about protein-protein interactions Jarek Meller Departments of Environmental Health and Biomedical Engineering, University of Cincinnati & Division of Biomedical Informatics, Cincinnati Children’s Hospital Research Foundation & Department of Informatics, Nicholas Copernicus University Joint work with Rafal Adamczak, Aleksey Porollo, Baoqiang Cao, Mukta Phatak, and Michael Wagner

JM - Can We Reliably Predict Interaction Sites from an Unbound Structure without Knowing Interaction Partners? Sequence only-based methods, structure-based approaches (e.g. hydrophobic patches, conserved hot spots), integrating structural and evolutionary info …

JM - From Sequence to Structure to Function: Which Residues Are Accessible to Solvent and Interaction Partners? Relative Solvent Accessibility of an amino acid residue in a protein quantifies the degree of exposure (surface exposed area, SEA) to solvent molecules in relative terms: RSA = SEA / MAX_SEA ; 0<= SEA <= MAX_SEA Thus, RSA is a real valued number in the interval [0,1], which for convenience may be scaled to take the values between 0% and 100%, where 0% corresponds to fully buried and 100% to fully exposed residues, respectively. / Folded conformation Extended conformation

JM - SABLE is a state-of-the-art RSA predictor MCCQ2 Adamczak et al., Proteins % Chen & Zhou, Proteins % Garg et al., Proteins % Liu et al., Proteins % “The two-state accuracy by SABLE is 77.3% in the ProSup benchmark, 77.9% in the SALIGN benchmark, 74.3% in the Lindahl benchmark and, 75.3% in the LiveBench 8 benchmark. This accuracy is consistent with the published performance of this and other state-of-the-art predictors.” Liu, Zhang, Liang and Zhou, Proteins 68 (2007) 47 CASP6 proteins; Garg, Kaur and Raghava, Proteins 61 (2005) 16 FR/NF CASP6 proteins; Chen and Zhou, Nucl. Acids Res. 33 (2005)

JM - Biases in RSA predictions for residues at the interaction interfaces: towards prediction of interaction sites Predictions obtained using SABLE; picture generated using the POLYVIEW server (A. Porollo) – also used to generate most animations and other pictures used in this presentation. Prediction “errors” at interaction interfaces: differences between predicted and actual (observed in an unbound structure) RSA values.

JM - Examples of SPPIDER predictions for proteins without homology to proteins used for training and validation … VHL Catalase CDK6 Red: known interaction sites predicted correctly; Blue: known interaction sites not predicted as such; Yellow: predicted sites without structural data supporting prediction