Case Studies Reference Library MultiChrome™ Microbiology Culture System Sensi-Ring™ Antibiotic Sensitivity System Case Studies Reference Library Kacey Diagnostics © 2010-14 All Rights Reserved

Sensi-Ring™ Antibiotic Sensitivity System Forward: This library is designed to help those using the Kacey Diagnostics C&S System with real world cases in which its unique chromogenic media has been used to identify bacteria and further discover its sensitivity to various antibiotics. These images were taken directly from the field and have not been retouched. This is a work in progress and are updating this library almost daily so please visit us often. We want to thank in particular Dr. Louis Gotthelf for his invaluable help in compiling this library and to the many hospitals and clinics using this system for sharing their findings with fellow colleagues. Any use of these images in a manor not approved by Kacey Diagnostics without its written consent is strictly prohibited.

INDEX: Type of Colony Pages Gram Negative Gram Positive 4 Ear Infections 5 UTI 6-7 Skin/Wound Mixed Colonies 8-10

Type: Gram Positive GP Patient/Breed: “Apollo”, 6 yr old Cocker Culture Source: Recurrent Ear/Skin infections MultiChrome Culture Results: Gram Positive: Staph/Strep both ears Sensitivity Results: Notes: Because these are both GP organisms, a GP Sensi-Ring was used. This is a very interesting culture because the Staph is sensitive to many of the common drugs including Penicillin. The Streptococci, however are resistant to enrofloxicin, which is typical for Streptococci. They do not usually respond to f luoroquinolones as is demonstrated here.

Type: Ear GN GP Patient/Breed: “Jessie”, Culture Source: R Ear MultiChrome Culture Results: Gram Negative: E. coli Gram Positive: Enterococci Sensitivity Results: Notes: Mueller Hinton plate was swabbed with both GP and GN material.

Type: UTI GN Patient/Breed: “Molly”, 19 yr old Feline Culture Source: Urine MultiChrome Culture Results: Gram Negative: E-coli Sensitivity Results: Notes: A Mueller Hinton plate was inoculated with the sediment as well. A Kacey UTI sensi ring was placed over the growth and incubated for 24 hours.

Type: UTI GN GP Patient/Breed: “Peaches”, 10 yr old M/Feline Culture Source: Urine MultiChrome Culture Results: Gram Negative: E-coli, Kleb Gram Positive: Enterococci Sensitivity Results: Notes: Only the Augmentin disk (amoxicillin/clavulanate ) and the Trimethoprim/Sulfa disk showed sensitivity to all bacteria. The Gentamycin (GM) disk showed sensitivity, but the zone of inhibition was too low to be therapeutic. .

Type: Mixed Infection GN GP Patient/Breed: “Baby”, Pomeranian Culture Source: Anal Glands MultiChrome Culture Results: Gram Positive: Enterococci Gram Negative: E-coli, Kleb Sensitivity Results: Notes: Antibiotic used “Ciprofloxacin

Type: Mixed Infection GN GP Patient/Breed: “Kizzy”, 3 yr old F Pug Culture Source: Pododermatitis MultiChrome Culture Results: Gram Positive: Staph & Strep Gram Negative: Pseudomonas &Kleb Sensitivity Results: Notes:K izzy has had demodex mange with pododermatitis for at least one year. There was so much swelling and exudation on the feet that the owner put socks on the dog’s feet to prevent licking. This dog has been treated for mange and bacterial skin infection by several different vets with no effect. On presentation, the feet were weeping purulent material. A culture was taken of the pus that was squeezed out of the skin lesions after the feet were disinfected in chlorhexidine.

Type: Mixed Infection GN GP Patient/Breed: “Zoe”, Maltese Culture Source: Anal Glands MultiChrome Culture Results: Gram Positive: Listeria Gram Negative: E.-coli Sensitivity Results: Notes: Antibiotic used “Clavamox”

“Kacey Products Providing Better Animal Care” 1854 A Hendersonville Rd Asheville, NC 28803 Ph: 1(828)685-3569 Fax: 1(828)685-3571 www.KaceyDiagnostics.com “Kacey Products Providing Better Animal Care” Kacey Diagnostics © 2010-14 All Rights Reserved