Systems Biology approaches to understanding glutathione metabolism 29 September 2010 Dr Najl Valeyev Edinburgh University of Kent Centre for Molecular Processing

Weather Electronic circuits design Aircraft flight controllers Stock market Different types of Systems

Causative knockouts of “functional” parts Lazebnik Y, Cancer Cell Sep;2(3):179-82

Linking parts with “disease” phenotypes

Family classification according to Phenotype

Idealised Cell as a System

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Science or Art ?

The Complexity Problem

Major Modelling Principle

True Object Knowledge 1 Knowledge 2 Knowledge 3 Knowledge 4

True Object Model 1 Model 2 Model 3 Model 4

Comparison of experiments with simulations Experiments Model predictions wt gshA- gshA-SpeA- SpeA-

Stochastic interactions between models and experiments should lead to deterministic conclusions  to test and evaluate the structure of the assumed intracellular interactions with the pathways  to identify inconsistent hypotheses and design experiments to clarify the properties of signalling pathways  to provide advanced interpretation of the experimental data  to developed an integrative model of fimbriation