Microbial Diversity Project leader: Daniela Goltsman

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Presentation transcript:

Microbial Diversity Project leader: Daniela Goltsman Jose Villanueva, Victoria Partida, Katherine Cantarero

Table Of Contents. . . Introduction Inoculation Isolation FISH Results Conclusion

Background Why are microorganisms important? How are microorganisms studied today? Aseptic technique Dyes What was the goal of our experiment? To study microbial diversity around our environment

Diversity of life Tree and 16s

Sampling Sites Our samples: Why did we choose these samples? Jose’s bathroom UC Berkeley’s Strawberry Creek Foothill Rec. Room Keyboard Katherine’s Dorm Why did we choose these samples?

Which location do YOU think is the diverse or “dirtiest” ?

What is inoculation? The introduction of microorganisms into any media. For us this process took about three days to complete. We incubated at 37 00 C

Jose’s Bathroom

Katherine’s dorm

Foothill Rec. room keyboard

UC Berkeley’s Strawberry Creek

Isolation Taking one colony or “population” of one type of microorganism and making those populations reproduce and grow. To our eye.

Isolate from Jose’s bathroom

Isolate from rec. room keyboard

UC Berkeley’s Strawberry Creek

What is FISH? FISH: Fluorescent In Situ Hybridization. This is a powerful technique that can be used to evaluate and analyze the presence of organisms in their community, their phylogeny, morphology, and number by targeting the 16S rRNA.

FISH probes What is a probe? Our dyes: Short sequence of DNA that hybridizes (binds) to a segment of the 16S rRNA. Each probe carries a different color dye. Our dyes: 4 different probes 3 different colors Blue- Gama proteobacteria & Eukaryotic cells Green- Universal bacteria Red- Beta proteobacteria

Sample 1 LAPTOP. Green: bacteria, red: beta-proteobacteria.

Sample 2: STRAWBERRY CREEK Green: universal bacteria, red: beta-proteobacteria, soft blue: gama-proteobacteria, bright blue: eukaryotes.

Sample 3:KATHERINE’S ROOM Red: beta-proteobacteria, blue: eukaryotes.

Sample 4. Green: universal bacteria, blue: eukaryotes

Conclusion In conclusion, we learned that: Microorganisms are EVERYWHERE! There are many kinds of bacteria that take on many shapes and sizes. Keyboards and bathrooms are the most diverse

Thank you!