Albia Dugger Miami Dade College Chapter 11 How Cells Reproduce

11.1 Henrietta’s Immortal Cells Henrietta Lacks died of cancer in 1951, at age 31, but her cells (HeLa cells) are still growing in laboratories HeLa cells are widely used to investigate cancer, viral growth, protein synthesis, the effects of radiation, and other processes important in medicine and research Understanding why cancer cells are immortal – and why we are not – begins with understanding the structures and mechanisms that cells use to divide

HeLa Cells

11.2 Multiplication by Division Division of a eukaryotic cell typically occurs in two steps: nuclear division followed by cytoplasmic division The sequence of stages through which a cell passes during its lifetime is called the cell cycle

The Life of a Cell Cell cycle A sequence of three stages (interphase, mitosis, and cytoplasmic division) through which a cell passes between one cell division and the next

Interphase Interphase consists of three stages, during which a cell increases in size, doubles the number of cytoplasmic components, and replicates its DNA G1: Interval of cell growth and activity S: Interval of DNA replication (synthesis) G2: Interval when the cell prepares for division

Interphase and the Life of a Cell Most cell activities take place during G1 Control mechanisms work at certain points in the cell cycle; some can keep cells in G1 Loss of control may cause cell death or cancer

Mitosis and Asexual Reproduction Mitosis is a nuclear division mechanism that maintains the chromosome number In multicelled species, mitosis and cytoplasmic division increase cell number during development, and replace damaged or dead cells later in life In asexual reproduction, a single individual can reproduce by mitosis and cytoplasmic division

Cell Division in Frog Embryos

Homologous Chromosomes Homologous chromosomes are pairs of chromosomes having the same length, shape, and genes Typically, one member of a homologous pair is inherited from the mother, the other from the father Human cells have 46 chromosomes (23 pairs) Except for a pairing of sex chromosomes (XY) in males, the chromosomes of each pair are homologous

Chromosomes During Division A cell can’t function without a full complement of DNA – each cell needs to have one copy of each chromosome The cell replicates its DNA in preparation for mitosis Four stages of mitosis (prophase, metaphase, anaphase, and telophase) parcel sister chromatids into separate nuclei Cytoplasmic division results in two diploid cells, each with the same number and kind of chromosomes as the parent

A An unduplicated pair of chromosomes in a cell in G1. Stepped Art B By G2, each chromosome has been duplicated. C Mitosis and cyto- plasmic division package one copy of each chromosome into each of two new cells. Figure 11-4 p179

Controls Over the Cell Cycle Most differentiated human cells are in G1 – some types never progress past that stage When a cell divides is determined by gene expression controls; some cause the cell cycle to advance, others stop the cycle from proceeding These built-in checkpoints allow problems to be corrected before the cycle proceeds

Figure 11-2 p177 prophase G2 metaphase S mitosis telophase anaphase G1 interphase

ANIMATED FIGURE: The cell cycle To play movie you must be in Slide Show Mode PC Users: Please wait for content to load, then click to play Mac Users: CLICK HERECLICK HERE

Take-Home Message: What is cell division and why does it occur? The sequence of stages through which a cell passes during its lifetime (interphase, mitosis, and cytoplasmic division) is called the cell cycle A eukaryotic cell reproduces by division: nucleus first, then cytoplasm; each descendant cell receives a set of chromosomes and some cytoplasm Mitosis is the basis of body size increases and tissue repair in multicelled eukaryotes; it is also the basis of asexual reproduction in single-celled and some multicelled eukaryotes Gene expression controls advance, delay, or block the cell cycle in response

11.3 A Closer Look at Mitosis When a nucleus divides by mitosis, each new nucleus has the same chromosome number as the parent cell The four main stages of mitosis are prophase, metaphase, anaphase, and telophase

Preparing for Mitosis During interphase, a cell’s chromosomes are loosened to allow transcription and DNA replication In preparation for nuclear division, chromosomes condense into their most compact “X” forms Tight condensation keeps the chromosomes from getting tangled and breaking during nuclear division

Interphase in Plant and Animal Cells Onion root cellWhitefish embryo cell

3D ANIMATION: Mitosis

Prophase Chromosomes condense Microtubules form a bipolar spindle Nuclear envelope breaks up Microtubules attach to the chromosomes Centrosome A region near the nucleus that organizes spindle microtubules; usually includes two centrioles

The Spindle Spindle A dynamic network of microtubules that forms during nuclear division Grows into the cytoplasm from opposite poles of the cell and attaches to duplicated chromosomes Microtubules from opposite poles attach to different sister chromatids and separate them

Metaphase and Anaphase Metaphase All duplicated chromosomes line up midway between the spindle poles Anaphase Microtubules separate the sister chromatids of each chromosome and pull them to opposite spindle poles

Telophase Two clusters of chromosomes reach the spindle poles A new nuclear envelope forms around each cluster Two new nuclei are formed, each with the same chromosome number as the parent cell

Mitosis

Take-Home Message: What is the sequence of events in mitosis? Each chromosome in a cell’s nucleus was duplicated before mitosis begins, so each consists of two DNA molecules Mitosis proceeds in four stages: prophase, metaphase, anaphase, and telophase During these four stages, sister chromatids of the duplicated chromosomes are separated and packaged into two new nuclei; each new nucleus contains the same number and types of chromosomes as the parent cell

11.4 Cytokinesis: Division of Cytoplasm In most kinds of eukaryotes, the cell cytoplasm divides between late anaphase and the end of telophase, but the mechanism of division differs between plants and animals Cytokinesis The process of cytoplasmic division

Cytokinesis in Animal and Plant Cells Animal cells A cleavage furrow partitions the cytoplasm A band of actin filaments rings the cell midsection, contracts, and pinches the cytoplasm in two Plant cells A cell plate forms midway between the spindle poles; it partitions the cytoplasm when it reaches and connects to the parent cell wall

Cytoplasmic Division in Animal Cells After mitosis is completed, the spindle begins to disassemble. At the midpoint of the former spindle, a ring of actin and myosin filaments attached to the plasma membrane contracts. This contractile ring pulls the cell surface inward as it shrinks. The ring contracts until it pinches the cell in two

ANIMATED FIGURE: Cytoplasmic division To play movie you must be in Slide Show Mode PC Users: Please wait for content to load, then click to play Mac Users: CLICK HERECLICK HERE

Cytoplasmic Division in Plant Cells The future plane of division was established before mitosis began. Vesicles cluster here when mitosis ends. As the vesicles fuse with each other, they form a cell plate along the plane of division. The cell plate expands outward along the plane of division. When it reaches the plasma membrane, it attaches to the membrane and partitions the cytoplasm. The cell plate matures as two new cell walls. These walls join with the parent cell wall, so each descendant cell becomes enclosed by its own cell wall

Take-Home Message: How do cells divide? After mitosis, the cytoplasm of the parent cell typically is partitioned into two descendant cells, each with its own nucleus In animal cells, a contractile ring pinches the cytoplasm in two In plant cells, a cell plate that forms midway between the spindle poles partitions the cytoplasm when it reaches and connects to the parent cell wall

11.5 Marking Time With Telomeres Telomeres protect eukaryotic chromosomes from losing genetic information at their ends Shortening telomeres are associated with aging

Telomeres The ends of eukaryotic DNA strands consist of noncoding sequences called telomeres Vertebrate telomeres have a short DNA sequence, 5′- TTAGGG-3′, repeated thousands of times Telomeres are important because a polymerase can’t copy the last hundred or so bases of the 3′ end of a chromosome When telomeres get too short, checkpoint gene products stop the cell cycle, and the cell dies

Stem Cells and Telomerase In an adult, only stem cells retain the ability to divide indefinitely Stem cells are immortal because they retain the ability to make telomerase, a molecule that reverses telomere shortening that normally occurs after DNA replication Research suggests that the built-in limit on cell divisions may be part of the mechanism that sets an organism’s life span

Telomeres

Take-Home Message: What is the function of telomeres? Telomeres at the ends of chromosomes provide a buffer against loss of genetic information Telomeres shorten with every cell division in normal body cells; when they get too short, the cell stops dividing and dies

11.6 When Mitosis Becomes Pathological On rare occasions, controls over cell division are lost and a neoplasm forms Cancer develops as cells of a neoplasm become malignant

Checkpoint Failure and Tumors When enough checkpoint mechanisms fail, a cell loses control over its cell cycle forms a neoplasm – a group of cells that lost control over how they grow and divide A neoplasm that forms a lump (abnormal mass) in the body is called a tumor

Oncogenes and Proto-Oncogenes An oncogene is any gene that helps transform a normal cell into a tumor cell Genes encoding proteins that promote mitosis are called proto-oncogenes – mutations can turn them into oncogenes Growth factors are molecules that stimulate a cell to divide and differentiate A gene that encodes the epidermal growth factor (EGF) receptor is an example of a proto-oncogene

Oncogenes and Overactive EGF Receptors

Tumor Suppressors Checkpoint gene products that inhibit mitosis are called tumor suppressors because tumors form when they are missing The products of the BRCA1 and BRCA2 genes are examples of tumor suppressors – they regulate the expression of DNA repair enzymes

Checkpoint Genes in Action

Cancer Benign neoplasms (such as ordinary skin moles) grow slowly, stay in one place, and are not cancerous Malignant neoplasms (cancers) disrupt body tissues, both physically and metabolically

Three Characteristics of Cancer Cells 1.Cancer cells grow and divide abnormally; capillary blood supply to the cells may increase abnormally 2.Cytoplasm and plasma membrane are altered; malignant cells typically have an abnormal chromosome number, and metabolism may shift toward fermentation 3.Cancer cells have altered recognition proteins and weakened adhesion – malignant cells break loose and invade other parts of the body (metastasis)

Figure p185 Benign neoplasms grow slowly and stay in their home tissue. Cells of a malignant neoplasm can break away from their home tissue. The malignant cells become attached to the wall of a blood vessel or lymph vessel. They release digestive enzymes that create an opening in the wall, then enter the vessel. The cells creep or tumble along inside blood vessels, then leave the bloodstream the same way they got in. They often start growing in other tissues, a process called metastasis

ANIMATED FIGURE: Cancer and metastasis To play movie you must be in Slide Show Mode PC Users: Please wait for content to load, then click to play Mac Users: CLICK HERECLICK HERE

Reducing the Risk of Cancer Each year, cancer causes 15 to 20 percent of all human deaths in developed countries Life style choices such as not smoking and avoiding exposure of unprotected skin to sunlight can reduce the risk of acquiring mutations that lead to cancer Some neoplasms can be detected with periodic screening procedures such as Pap tests or dermatology exams

Skin Cancers A Basal cell carcinoma is the most common type of skin cancer. This slow- growing, raised lump may be uncolored, reddish- brown, or black. B The second most common form of skin cancer is a squamous cell carcinoma. This pink growth, firm to the touch, grows under the surface of skin.

Take-Home Message : What is cancer? Cancer is a disease that occurs when the abnormally dividing cells of a neoplasm physically and metabolically disrupt body tissues A malignant neoplasm results from mutations in multiple checkpoint genes Although some mutations are inherited, life style choices and early intervention can reduce one’s risk of cancer

Video: Genetics, Sociology, and Breast Cancer