Study on application of Chinese Biological Pesticide Speaker: Kelley Zhou Beijing Multigrass Formulation Co.,Ltd

Overview of biological pesticides Introduction of popular biological pesticides Problem and suggestion of biological pesticides

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 选择性强 Selective 4  对生态环境影响小 Environmental friendly  产品寿命长 Long life of products  原材料来源广泛 Rich Raw Material

1. 应用会更加广泛 Extensive Application 2. 市场份额会逐年增加 More Market Share 3. 和化学农药共同发展，相辅相促 Coexist with Conventional Pesticides 5

Advantages of Chinese Bio-pesticides 6 1. 几千年的历史经验 Historical experience of thousands years 2. 雄厚的理论和技术基础 Deep theoretical and technical foundation 3. 广泛的原材料来源 Extensive Source of Raw Material

7 Types of active ingredients More than 80 Numbers of products More than 2500 Yearly capacity of formulation More than 100 thousand MT Application area billion acres 7

EuphorbiaStemonaTripterygiumMelia toosendan Croton Sophora flavescens Main products in the market

井岗霉素 Validamycin 阿维菌素 Abamectin 赤霉素 Gibberellin 苏云杆菌 BT 9 Main products in the market

Matrine Matrine is a kind of alkaloid extracted from Sophora flavescens Ait. It can act as pestidide, fungicide and also stimulating plant growth and used for controlling the pests of Lepidoptera, mites,etc.alkaloidLepidoptera Matrine

Activities and features of Sophora flavescens Ait extract 1.Insecticidal activity contact poison firstly, stomach poison secondly. It can control and kill pests. 2. Bactericidal activity It can inhabit fungus, bacterium and virus. 3. Stimulate plant growth Amino acids, fatty acids and other substances in it can stimulate and regulate the growth of crops.

Field trial collection of Matrine product

Test Condition Test place: Rio Grande do Sul in Brazil Temperature: 17,9°C - 23,8° Humidity: 73%---87% Test period: October 2008 Test design Treatment: 0.5% matrine SL 1000 times, 40% omite EC 1000 times and water control Dosage: 1kg/ha for each concentration Application times: one application for each treatment Efficacy calculation We check the pests alive and dead number after 1 day, 2days, 3days and 5 days. Test Condition Test place: Rio Grande do Sul in Brazil Temperature: 17,9°C - 23,8° Humidity: 73%---87% Test period: October 2008 Test design Treatment: 0.5% matrine SL 1000 times, 40% omite EC 1000 times and water control Dosage: 1kg/ha for each concentration Application times: one application for each treatment Efficacy calculation We check the pests alive and dead number after 1 day, 2days, 3days and 5 days.

The mites observed on the leaf Mites zoomed in

Three days after application of 40% Omite 1000times Three days after application of 0.5% matrine SL 1000times

controlled by 0.5% matrine SL Control by omite 40% EC

Test Condition Test place: an indoor laboratory of Bogota City in Colombia Test period: August 2006 Test design Treatment: 0.3% matrine 1000 times(1kg/ha), 20% methomyl 1500 times(0.75kg/ha) and water control Application times: one application for each treatment Efficacy calculation We check the pests alive and dead number after 1 days, 3 days, 7 days and 10 days. For each check, we chose 10 plants randomly and for each plant we checked 10 leaves. Test Condition Test place: an indoor laboratory of Bogota City in Colombia Test period: August 2006 Test design Treatment: 0.3% matrine 1000 times(1kg/ha), 20% methomyl 1500 times(0.75kg/ha) and water control Application times: one application for each treatment Efficacy calculation We check the pests alive and dead number after 1 days, 3 days, 7 days and 10 days. For each check, we chose 10 plants randomly and for each plant we checked 10 leaves.

It is in threshold before application The bud covered by aphides

3 days after application of 0.3% matrine1000 times

Oligo Saccharin Based on halobios ， advanced technology extract the low- molecular carbohydrate The animals & plants self immune formulation Supply nutrition Prevent and cure disease of crop Human medicine, plants care, plants nutrition

Improve solid environment Induce plants to generate disease- resistant hormone and active cells Provide plants nutrition Function Target diseases: Downy Mildew, Fusarium Wilt, Verticillium Wilt, Ring Spot, Anthracnose, Angular leaf spot, soft rot, Root knot nematode, etc. Target diseases: Downy Mildew, Fusarium Wilt, Verticillium Wilt, Ring Spot, Anthracnose, Angular leaf spot, soft rot, Root knot nematode, etc.

Product: 3% Oligo sacchrin Disease ： downy mildew Level of disease ： moderate Crop ： rose Date ： 06/12/ /12/2007 Method ： foliar spray, 7 days interval, 3 times Dosage ： 1.5L/ha.,dilution rate is 600 Person in charge: Hernando Location ： Colombia Contrast: metalaxyl

The disease was controlled after applied with 3% Oligo saccharin

The disease was still alive after applied with metalaxyl.

Suggestion: When crops infected by downy mildew, dilute Baiclean with 600 time, foliar spray. 7 days interval, 3 times.

Use of Oligo saccharin at the beet crop

 Objective: To evaluate performance in the control of nematode Products used: 3% Oligo saccharin x Nemix(nematocide) x Control Technical Responsible: Ricardo Martins Property: Farm Canada City: Uberaba, State of Minas Gerais Producer: Claudio Carvalho Ottoni Date of installation of test: 18/12/2008  Pontecial of the producer: Carrot 650 ha to 700 ha / year Beet 120 ha / year  Culture: Variety: Beet Ferry Morse ： Ferry Morse Destination of output: Fresh Consumption  Date of sowing / transplanting: 18 / 12 / 08  Date of collection: 08 / 03 / 08 Planting spacing: plots has a width of 1.75 m with 4 double rows, where we have 20 plants in double line, obtaining a final stand of 457,000 plants / ha.

 Technique of cultivation: Irrigation Central Pivot.  Methodology: The trial was to test an area of 1,000 m 2 and the control is in the central area.  The applications were as follows: – 1st application 03 days after sowing; – 2nd application 07 days after germination; – 3rd application 20 days after germination; – 4th application 40 days after germination; – 5th application 60 days after germination. All applications were isolated, because we do not know the compatibility, and used for all applications a flow of 400lts of water / ha with a dosage of 800ml/ha. Valuation: - 1st Rating: 19/01 - after thinning - 2nd Rating: 17/02

Control (without nematicide)

NEMIX (Standard) One Application 3% oligo saccharin Four Application

15% Bioflavonoid Complex Extracted from orange pill Crops: vegetable, horticulture and fruit. Control bacterial and fungal diseases

Test information Product Contrast Disease 15% Bioflavonoid Complex Kresoxim-methyl Powdery mildew Disease level Crop Date Moderate Rose 23/03/ /04/2009 Method Dosage Person in charge Foliar spray L/ha., dilution rate Murad Location Ethiopia

Overview of rose plantation

When the buds of rose infected by powdery mildew, they would not open or even they will not come into flower.

New leaves sprouted after applied with 15% Bioflavonoid Complex. After recovery, the white powder was removed, there were yellow spot on the leaves.

Test result

46 1. 认知的误区 ( acknowledge) 2. 生产污染 ( Pollution) 3. 使用方法 ( Application ) Problems

47 1. 发掘历史沉淀 (Histrorical resource) 2. 重视制剂的研发 (Formulation) 3. 加大登记的投入 (registration)