Effects of Drugs on Daphnia Michelle Dilgard Undergraduate Student Education Major Biology Concentration Tennessee Technological University Cookeville,

Slides:

Advertisements

Similar presentations

Materials taken from Knisely, Karin

Advertisements

Bioassay Investigations with Daphnia Developed by Kathy Cahill Wantagh High School Wantagh, New York.

The LD50 of Common Household Toxins on Brine Shrimp

The Effects of Caffeine on Plant Germination

Notebook for October 2nd Pick up your Folder and staple two lab sheets together Reflect yesterday Write: Today’s Date Write down Essential Question (Explain.

Figure 1. Average percent change in heart rate.

The Effects of Caffeine and Triclocarban on Gammarus pseudolimnaeus Jim Fietzer Department of Biological Sciences, University of Wisconsin – Whitewater.

Susceptibility of Antibacterial Chemicals on Pseudomonas aeruginosa Research performed by: Clayton Brady Health Science Biology Major Tennessee Technological.

Roadway pollutant effects on abundance of Odonate nymphs in a wetland of the Eastern Highland Rim, Tennessee Jason Payne, Undergraduate Student (Marine.

S1 Microscope IDL Project. Example images Fabric Daphnia Font 10 print.

INTRODUCTORY CHEMISTRY INTRODUCTORY CHEMISTRY Concepts and Critical Thinking Sixth Edition by Charles H. Corwin Chapter 1 1 © 2011 Pearson Education, Inc.

By: Eric Nam.  Neurons: A specialized cell transmitting nerve impulses; a nerve cell  Neurotransmitters: A chemical substance that is released at the.

Mouthwash Effectiveness in Eliminating Bacteria By: Matthew Mancuso Department of Biology Tennessee Technological University, Cookeville, TN

Effects of Drugs on Daphnia

Effects of Nicotine on the Pulse Rate of Lumbriculus Variegatus

By: Kayleigh Makoid.  Nicotine  Epinephrine  Daphnia  Previous testing.

Effect of Environmental Conditions on Heart Rate: Experimenting with Daphnia AP Biology Lab 10.

Physiology of the Circulatory System AP Biology Lab 10.

Effects of Drugs on Heart Rate Introduction My experiment deals with the affects of different drugs on the heart rate of daphnia. Drugs I will use include:

The Effects of Directional Drilling Fluids on the Environment Shannan Casteel Undergraduate Student (Professional Communication Concentration) Department.

Scientific Research in Biotechnology 5.03 – Demonstrate the use of the scientific method in the planning and development of an experimental SAE.

Vitamin C: Does It Prevent The Common Cold? Jessica McCormick Undergraduate Student Department of Biology (Health Science Concentration) Tennessee Technological.

COMPETITION PROJECT Larry G. Cabral, Nelida Rojas GK-12 Santa Ana High School.

TOPIC: Scientific Method AIM: How can we organize data collected from an experiment? DO NOW: Does the price of sneakers affect how high a person can jump?

The Effect of on the Heart Rate of Daphnia magna List all team members here.

Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.

How to Write a Formal Lab Report. Why do we write lab reports?  Essential to clearly communicate how the lab was conducted and what the findings were.

Integrated Science Day 8 September 24, Learning targets Learning targets 1. I can evaluate a laboratory procedure and analyze the data to form a.

By: Carrie Bidwell (Clay High School) & Emily Zablocki (Adams High School)

The Effects of Light on Tagetes patula Growth Amber D. Jones Undergraduate Student Zoology Concentration Dept. of Biology, TTU Cookeville, TN

Boric Acid and Lumbriculus Variegatus Jonathan Trembley and Michael Welles, GCC Bio 100 Introduction: Lumbriculus Variegatus is a fresh water worm found.

How Effective Iodine Is Against Acanthamoeba Paul E. Seibers III Undergraduate Student (Microbiology Concentration) Department of Biology Tennessee Technological.

Scientific Cents. Making Observations Work with your partner Read the procedure carefully. Make a data table and a data chart to record your observations.

The effects of Alcohol on the pulse rate of the Lumbriculus Variegatus By: Mallory Broda & Andrea Aman Introduction : Lumbriculus variegatus, also known.

Effects of temperature on the growth of goldfish (Carassius auratus).

Metabolic Pathways of Plants Nathan J. Smith Undergraduate in WFS Tennessee Technological University Cookeville, TN

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

Toxicology Final Presentation Dyad 1 Hassanain A Sola A.

September 18, 2012 Check the clipboard for your new seat I NTEGRATED S CIENCE D AY 6.

What is a Bioassay?. Bioassay   A bioassay involves the use of biological organism to test for chemical toxicity. Canaries are used by coal miners as.

The Effects of Valerian Root on the Pulse Rate of Lumbriculus variegatus By: Jacob Squicciarini and Isabella Cox Albion High School GCC Biology 100 Variables.

Procedure: 1.Collect two groups of five worms in separate containers using two pipettes, one per container. Introduce stimulant to one container and spring.

September 14, 2012 I NTEGRATED S CIENCE D AY 5. L EARNING TARGETS 1. I can write a scientifically based conclusion 2. I can perform an experiment on a.

Unit 3 Investigative Biology. SQA Success Criteria  Explain the need for a pilot study in terms of the dependent variable, the independent variable,

Properties and changes of materials Grade 5 science.

Effect of Analgesic Medications on the Heart Rate of Daphnia magna Bin Huang Aberdeen Central High School Purpose Hypothesis Methods Discussion H ₁ : The.

Effect of Caffeine on Lumbriculus variegatus By: Monica Benjovsky and Kellie O’Hearn Introduction: What is the effect of caffeine on a Blackworm? This.

Wednesday, February 17, 2016 What are we doing today? Entry Task

What Are You Drinking, TEA? Tira J. Robinson Junior Microbiology Major Tennessee Tech University.

Figure 1. Average percent change in heart rate.

Blackworm Toxin: Ibuprofen

Mean body size of female Daphnids

Outline Bioassay background (5 mins)

Pulse of Blackworm in Caffeine Solutions

How Do Scientists Conduct Controlled Experiments?

Investigations with Membrane Transport

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

The effect of depressants on the heart rate of Lumbriculus variegatus

The Effects of Caffeine on the Heart Rate of Daphnia magna

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

Chapter 1 Introduction to Chemistry by Christopher Hamaker

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

Chapter 1 Introduction to Chemistry by Christopher Hamaker

Blackworm Lab: average pulse rate

Chapter 1 Introduction to Chemistry by Christopher G. Hamaker

The effect of a depressant on the Bpm of

Daphnia Inquiry Lab Ms. Kluesner AP Biology.

Presentation transcript:

Effects of Drugs on Daphnia Michelle Dilgard Undergraduate Student Education Major Biology Concentration Tennessee Technological University Cookeville, TN 38505

Project Summary Problem: How do drugs effect Heart Rate? Using Daphnia is more appropriate Through the performing of an experiment

Introduction andBackground Introduction and Background Daphnia –Water Flea (Wong, 1997) Many studies have been done previously Drugs to be used –Caffeine (Foster, 1997) –Nicotine –Alcohol –Ibuprofen (Cluevers, 2003)

Objective I will observe the changes in heart rate of Daphnia when exposed to Caffeine, Ibuprofen, Alcohol, and Nicotine. I will have several separate samples of Daphnia in my study. Each community will be exposed to a different drug and observe how the heart rate of the Daphnia changes accordingly.

Hypothesis I believe the Daphnia heart rate will increase when the Daphnia are exposed to Caffeine and Nicotine. I believe the Daphnia heart rate will decrease and slow down when the Daphnia are exposed to Ibuprofen and Alcohol.

Methods Label containers Place Daphnia on slide Record Heart Rate Add experimental solution Record new heart rate Repeat Data Form

Materials Water Depression Slides Cover slips Droppers Daphnia Magna Microscope Watch Petri Dishes Daphnia Anatomy Chart Cigarettes 80 proof vodka No-dos Ibuprofen tablets

Expected Results I expect that the Daphnia’s heart rates will increase with Nicotine and Caffeine because both of these drugs are stimulants. I also expect that the Daphnia’s heart rates will decrease with Alcohol and Ibuprofen solution.

Benefits This experiment will show not only the effects of these drugs on Daphnia but also what they similarly do to the human body when it is exposed. Daphnia are used as a humane alternative to Humans when performing this type of experiment.

Timeline ~ Title Due ~ Literature Reviews and Works Cited Due ~ Method Objective and Hypothesis Due ~ Research Proposal ~ Website ~ Midterm Due ~ Begin Experiment ~ Final Presentation

Budget Petri Dishes- $5.10 Slides- $7.50 Cover Slips- $3.20 Daphnia- $10.95 Caffeine Pills- $7.49 Ibuprofen- $9.99 Cigarettes- $3.40 Vodka- $16.99 Pipettes- $10.00 TOTAL= $74.62

Works Cited Campbell, Anthony K., Kenneth T. Wann, and Stephanie B. Matthews Lactose causes heart arrhythmia in the water flea Daphnia pulex. Comparative Biochemistry and physiological Part B Biochemistry and Molecular Biology 139: Cluevers, Michael Mixture toxicity of the anti-inflammatory drugs diclofenac, ibuprofen, naproxen, and acetylsalicylic acid. Exotoxicology an Environmental Safety 59: Foster, Rachel A stroboscopic method to investigate the effect of caffeine on Daphnia hear rate. Journal of Biological Education 31: Wong, Diana C.L., Philip B. Dorn, and Eric Y. Chai Acute toxicity and structure-activity relationships of nine alcohol ethoxylate surfactants to fathead minnow and Daphnia magna. Environmental Toxicology and Chemistry 16: Wang, Helen Ying, Allen W. Olmstead, Hong Li, and Gerald A. LeBlanc The screening of chemicals for juvenoid-related endocrine activity using the water flea Daphnia magna. Aquatic Toxicology 74: Villegas-Navarro, Arturo, Esperanza L. Ross, and Jose L. Reyes The heart of Daphnia magna: effects of four cardioactive drugs. Comparative Biochemisty and Physiology Part C Toxicology and Pharmacology 136C: