Expression Data Generation and Collection at MPIMG and RZPD Resource Center within the German Human Genome Project Steffen Schulze-Kremer

Background History  1987 first spotting system (EMBL,ICRF)  ~1989 complex cDNA Hybridisations to arrays  ~1989 onward distribution of arrays in reference library system

RZPD Database Objectives (since 95)  To support dissemination of variety of biological materials Clones Filters Pools  To collect and redistribute biological primary data Hybridisations Sequences Phenotypes

RZPD Database Coverage  Biological Information Clones Libraries Genes Sequences Hybridisation  Logistic Information Clone storage Library re-arraying Filters  Accounting Information Customers Orders / requests Affiliation (DHGP?) Price lists   Information Integration Confidential information Links to other databases Query Visualisation

RZPD Database Contents (Oct 5/99)  Biological Information clones master clones 319 libraries filters sequences hybridisations Blast hits distinct targets hit  Accounting Information 5551 registered users 1228 organisations 630 German 598 foreign # RZPD orders (in RLDB) clones (4405) 3610 filters (1468) 1118 pools (0)  Submissions sequences (DHGP) sequences (other)

Graphical Filter Display Tool

Database Components  Oracle / Unix  DEC Alpha 8 Processor Server  Secure Web Server protects confidential information during transit different roles available customised views  OPM Tools some 80 object classes class browser query editor

OPM Schema Browser

Hybridisation Data in RZPD

Laboratory Database at MPIMG

Dictionaries

„Owned Objects“

„Result“ „Experiment“ „Result“ „Experiment“  Lab Database Development Team at MPIMG Dr. Arif Malik, Axel Nagel, Dr. Holger Eickhoff

RZPD Distribution Service, Database & Bioinformatics Team (  Distribution Service Nadja Pohl, Eva Partsch, Patience Frederick, Maryam Sabet  Registration Radka Kochan  System Administration Klaus Dobrindt, Robert Fürst  Data Submission Dr. Andreas Hewelt  Protein Structure Factory Dr. Jörn Quedenau, Ursula Meyhoefer  Database Managers Karsten R. Heidtke, Peter Neubert  Web Designer Iris A. Bertram  Application Programmers Elisabeth Maschke, Ralf Mühlhaus  Students Falco Ripsas, Katja Wegner  Informatics Manager Dr. Steffen Schulze-Kremer