The Potato Catalase Pre-Lab. 100 g of potato were cut into small chunks Potato Catalase Experiment.

Slides:

Advertisements

Similar presentations

Water Titration and You In this presentation, you will learn how to prepare your daily dose using the water titration method.

Advertisements

PH, Acids and Bases.

Unit 2 PPA3 The effect of changes in pH and temperature on the decomposition of hydrogen peroxide.

Extraction of “clay fraction”.

Carolina kit # $ refill # $59.95 Deb Semmler UNDERSTANDING PROPERTIES OF MATTER.

Ammonium Test. 1. Draw a line 5 mm from the top of the cuvette.

HIGHER CHEMISTRY REVISION. Unit 1:- Reaction Rates 1. A student was asked to write a plan of the procedure for an investigation. The entry made in her.

CHEM 1031 Stoichiometric Ratio. Procedure PUT GOGGLES AND APRONS ON. Equipment Needed (per pair): (2) 400 mL beakers (2) 100 mL graduated cylinders (1)

South Carolina Biology Standard 2.8

Titration Pre-lab.

Acids, Bases, pH and Buffers. Acids An acid dissolves in water to donate H + In water the H+ reacts with water to make the hydronium ion H + + H 2 O 

Enzymes PHOTOSYNTHESIS:CO 2 + H 2 O → Glucose + O 2 RESPIRATION:Glucose + O 2 → CO 2 + H 2 O How do these processes happen? What do the arrows mean? Hmm!!

Investigating Catalase Topic 3 IB Internal Assessment.

4.3 (a) To investigate the effect of pH on amylase activity

Steps for Water Displacement Volume Measurements Using a Graduated Cylinder.

BANANA DNA EXTRACTION.

ISOLATION OF DNA FROM ONIONS 植物 DNA 的粗提. Aim To isolate nucleic acids from onion tissue. Note: nucleic acids prepared in this way will not be very pure.

Gas Liquid Liquid Gas The three common states of matter are:

Lab Equipment Biology Foothill Technology High School.

Lab: Investigating pH of Various Household Products

Red Cabbage Extract as a pH Indicator. Aim Extracting a pH indicator from the red cabbage. Finding the color of the extract to a known pH solution. Determining.

CAPT Lab: Investigating the Effect of Temperature on Enzyme Activity Created by Mr. Cross

Enzymes. Enzymes are Proteins Many of our genes code directly for enzymes Estimated to be about 75,000 different types in the human body.

Measure 25 ml of red water and pour it into test tube A. Step 1 Remember 25 is 5 lines above the 20.

DNA in My Food???. What is DNA ? Where do you find DNA?

Biology Ms. Haut. 1. Add 1 ml (10 drops) of Benedict’s solution to the test tube. 2. Add 8 drops of sample solution to a test tube. 3. Heat the mixture.

4.6 To prepare one enzyme immobilisation and examine its application

Using a Graduated Cylinder. 1. Understand the size of the graduated cylinder and its markings- 100:1ml near the top means it is a 100 milliliter (ml)

High Sucrose Soybean Thin-Layer Chromatography (TLC)

“ Chemical Changes/Reactions” Investigation # Materials used (reactants) Observations You should use several lines for each row of your table.

For each scenario below: 1) Identify the independent variable, levels of change, dependent variable, number of trials, constants, and control(s). (if present)

Fruit Cup DNA Extraction. Step 1 Into one of the 5 oz cups add 1 teaspoon of shampoo. Fruit Cup DNA Extraction.

Lab Report 2 Does It All Add Up?. Title Page Name of Lab, Name of Student, Date, Period, Subject Name of Lab: Does It All Add Up? The Rest should be self.

Student Directed Investigation – due dates. Due Dates February 13 – Methods & Materials Section due (in Google drive – shared with me) February 13 – Introduction.

Topic 4 - Properties of Enzymes and Uses in Industries National 4 Cell Biology.

Enzyme Catalysis Lab. Materials – at lab station 20 small cups 3 large cups 4 syringes Piece of white paper Dropper Glass beaker Test tube Test tube holder.

Phase Two Titration Year 10 EEI by Mr H Graham Volumetric analysis is; A type of chemical analysis which depends on the accurate measurement of solution.

4.3 (b) To investigate the effect of pH on catalase activity.

Elephant Toothpaste.

4.10 To isolate DNA from a plant tissue

Cell metabolism and Enzymes. Metabolism (all of the chemical reactions in a living thing) Anabolic reactions Catabolic reactions Small molecules are combined.

Elephant Toothpaste.

Rates of Reaction In this unit you will investigate what makes chemical reactions go faster. At the end of the unit you will have a 2 hour practical test.

General Biology lab Lab 3 Enzymes.

C DOES PH AFFECT THE RATE OXYGEN IS RELEASED? By: Bekah, Emily, Miranda, & Savana.

Investigating Catalase

ACIDS AND BASES REVISION Q3. A STUDENT HAS THREE UNLABELLED BEAKERS EACH CONTAINING A COLOURLESS LIQUID. ONE CONTAINS WATER, ONE CONTAINS A SOLUTION OF.

Wine room Ferric casse In this experiment we determined the amount of iron in wine. - The process of the experiment - Measurements - Conclusion.

TODAY Put the following table and problem below on a piece of paper

Enzyme Reactions in Living Organisms

En Route Edible Bug Juice Abdul Rahman Banisheyba and Hudhaifat Hamdan

*Also get out Assignment #47 (pH WORKSHEET) to be stamped.

Vitamin C Titration Group Presentation

Investigating Catalase

Catalysis Experimental Design

Before you start get or make a copy of this Table of results

Extraction of “clay fraction”.

Fruit Cup DNA Extraction.

Before you start get or make a copy of this Table of results

Biochemical Methodology

Investigating Cells Enzymes Mr G Davidson.

Analysis of an Acid Base Titration Curve the Gran Plot

HIGHER CHEMISTRY REVISION.

4.10 To isolate DNA from a plant tissue

Analysis of an Acid Base Titration Curve the Gran Plot

10/19/2011 Objective: Understand pH and concentration Design a lab procedure Warm-Up: You have two test tubes. Each has 10 mL of hydrogen peroxide solution.

Action of urease Objectives

By: Sam, Christiane, and Kayla

pH: Hands on strategies to tackle misconceptions

Presentation transcript:

The Potato Catalase Pre-Lab

100 g of potato were cut into small chunks Potato Catalase Experiment

The chunks were put into a blender along with 200mL of water Potato Catalase Experiment

The mixture was then pureed at high speed for 1 minute Potato Catalase Experiment

The puree was then poured through a wire sieve Potato Catalase Experiment

The sieve separates out the foam and the larger chunks of potato Potato Catalase Experiment

The foam is then discarded Potato Catalase Experiment

Household vinegar and ammonia were used to adjust the pH of the potato extract Potato Catalase Experiment

A 25mL graduated cylinder was used to measure out 6mL of potato extract which was then added to each test tube Potato Catalase Experiment To ensure valid results, the graduated cylinder was rinsed out between measurements

Drops of vinegar or ammonia were then added to each test tube to obtain the desired pH as shown in the table below Potato Catalase Experiment pH 6pH 7pH 8pH 9 Vinegar2 drops0 drops Ammonia0 drops 2 drops4 drops Distilled Water2 drops4 drops2 drops0 drops

3mL of hydrogen peroxide (3%) were then poured into each of four smaller test tubes Potato Catalase Experiment

The 3mL samples of hydrogen peroxide were then added to each test tube and stirred for 2 seconds Potato Catalase Experiment To improve validity, H 2 O 2 was added working left to right, and then each mixture stirred working left to right.

After 2 minutes, a marking pen was used to indicate the upper and lower limits of the foam columns. Potato Catalase Experiment To make measurements valid, the marking was also done working left to right.

The data table below shows the results of 3 trials. Potato Catalase Experiment Potato Catalases & Hydrogen Peroxide Foam Heights (mm) Trial 1Trial 2Trial 3Average pH pH pH pH Three trials helps make the results more reliable. More trials would be even better.

Foam Height (mm) as a function of the effect of pH on Catalase Activity Potato Catalase Experiment pH Average Foam Height (mm) mm 24.3mm 14.0mm 27.7mm