Amino Acid Enzymes Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase.

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Presentation transcript:

Amino Acid Enzymes Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase

Amino Acid Structure

Amino Acid Enzymes Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid

Peptone Iron Agar Deeps Peptone – protein rich in cysteine Iron - Fe

Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid H2S Fe (Iron) FeS (Ferrous Sulfide) Black

Cysteine desulfhydrase

Amino Acid Enzymes Lysine decarboxylase Lysine Cadaverine + Carbon dioxide

Lysine Decarboxlyase Broth Lysine Fermentable sugar ph indicator Basic = purple Acid = Yellow Durham Tube Mineral Oil on top after inoculation (maintain an Anaerobic environment)

Lysine decarboxylase broth

Amino Acid Enzymes Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia

Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia FeCl2 (Ferric Chloride) HCl Green

Phenylalanine Agar Slants

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