GSCI 2013: Group 2-Cell and Developmental Biology Seth Jones, University of Kentucky Karen Maruska, Louisiana State University Joy Davis, Baton Rouge Community College Sabrice Guerrier, Millsaps College Naila Mamoon, Millsaps College Facilitator: Peter Cavnar, University of West Florida

CELLULAR RESPIRATION

Context What kind of course is unit designed for? mid-level How long is unit? 1 week When will the unit be used in the course? After students have learned basic chemistry, energetics, redox rxn, protein structure, cell membrane, transport, and organelles Class size? Should be fine for all ranges

Learning goal Understand how the energy from electrons in glucose is converted to chemical energy in ATP during oxidative phosphorylation.

Explain the role of oxygen in cellular respiration Explain how the H+ gradient across the inner mitochondrial membrane is generated and how it drives ATP synthesis Predict the effects of various drugs on oxidative phosphorylation Learning outcomes

How long can you hold your breath?

Glucose  Heat C 6 H 12 O 6 O2O2 CO 2 H2OH2O ATP Loss of hydrogen atoms (becomes oxidized) Gain of hydrogen atoms (becomes reduced) Brainstorm: So why do you think we need oxygen?

NADH FADH 2 ATP CYTOPLASM Glycolysis Electrons carried by NADH Glucose Pyruvate Pyruvate Oxidation Citric Acid Cycle Oxidative Phosphorylation (electron transport and chemiosmosis) Mitochondrion Substrate-level phosphorylation Oxidative phosphorylation

Figure 6.10 Oxidative Phosphorylation Electron Transport Chain Chemiosmosis Mito- chondrial matrix Inner mito- chondrial membrane Intermem- brane space Electron flow Protein complex of electron carriers Mobile electron carriers ATP synthase NADH NAD  2 H  FADH 2 FAD O2O2 H2OH2O ADP PATP 1 2 HH HH HH HH HH HH HH HH HH HH HH I II III IV

Welcome to Club Mitochondria Instructions: Pair with a partner then identify the characters within the analogy that correspond to the following: 1. Electrons 2. Protons 3. Electron transport chain (ETC) 4. ATP synthase 5. Mitochondrial Matrix 6. Intermembrane space

What is the layout in Club Mitochondria? Foyer Dance Floor Main entrance Female Patron Male Patron

Consider a scenario where the electron transport chain ("dance line") is disrupted in the mitochondria. What is most likely to happen to the H+ concentration ([H+]) in the intermembrane space? A. Decrease in [H+] B. Increase in [H+] C. No change D. I don't know

Consider a scenario where the electron transport chain ("dance line") is disrupted in the mitochondria. What change would you expect in ATP concentration in the cell? A. Decrease in [ATP] B. Increase in [ATP] C. No change D. I don't know

Figure 6.11 ATP synthase NAD  NADH FADH 2 FAD HH HH HH HH HH HH HH HH 2 H  H2OH2O ADP ATP P O2O2 2 1 Rotenone Cyanide, carbon monoxide Oligomycin DNP Your homework: List the signs and symptoms in victims with acute cyanide poisoning AND we would like you to extend the analogy to include oxygen and ATP Inhibitors of cellular respiration