VISIT IN TUBINGEN. The retinal preparation process 1. Removing the eye cup of rat 2. Cut the eye cap along the ora serrata 3. Dissect the retina for three.

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Presentation transcript:

VISIT IN TUBINGEN

The retinal preparation process 1. Removing the eye cup of rat 2. Cut the eye cap along the ora serrata 3. Dissect the retina for three segments that will be recorded 4. Loosen the neural retina from the sclera and transfer it to the recording field of the MEA

The retinal preparation process

The perforated MEA  Improve retina - electrodes contact. Measure the ganglion cell activity.

The perforated MEA setup  The improved contact achieved by applying a negative pressure fro underneath the MEA

MCS system  Amplifier and data acquisition.  MEAs.  Light sources to stimulate the retina.  Perfusion setup.

STIMULUS OF UNIFORM LIGHT Light intensity of 3.6klux Cycle time Total time of 30 sec TP=time of pulse f= frequency.

The f and TP values 5ms50ms100ms500ms 0.25Hz 0.5Hz 1Hz 5Hz 10Hz 20Hz

The signal processing block diagram FFT TRANSFORMATION ROW ELECTRODE FILTER 200Hz-3kHz reconstruction Frequency space Frequency space (peak detector) Level filter 50mv Time space Process the date (frequency, number of AP, width of the respond …)

Compare between 50,100,500 ms pulse at 0.5Hz from one electrode number 47

Frequency space

Frequency space zoom in

Frequency space filtered

Reconstructed signal 50,100,500 ms with peak level at 50mV

50ms action potential counter

100ms action potential counter

500ms action potential counter

50ms Action potential frequency

100ms Action potential frequency

500ms Action potential frequency

Compare between 50,100,500 ms pulse at 0.25Hz from one electrode number 47

Frequency space

Frequency space zoom in

Frequency space filtered

Reconstructed signal 50,100,500 ms with peak level at 50mV

50ms action potential counter

100ms action potential counter

500ms action potential counter

50ms Action potential frequency

100ms Action potential frequency

500ms Action potential frequency

The total result of all the electrode stimulus of 0.5Hz 50ms

Number of action potential at 10ms stimulus of 0.5Hz 50ms

Action potential frequency (calculated at 100ms) stimulus of 0.5Hz 50ms

The total result of all the electrode stimulus of 0. 5Hz 100ms

Number of action potential at 10ms stimulus of 0.5Hz 100ms

Action potential frequency (calculated at 100ms) stimulus of 0.5Hz 100ms

The total result of all the electrode stimulus of 0.5Hz 500ms

Number of action potential at 10ms stimulus of 0.5Hz 500ms

Action potential frequency (calculated at 100ms) stimulus of 0.5Hz 500ms

Zoom in at the stimulus of 0.5Hz 500 ms pulse counter.

Zoom in at the stimulus of 0.5Hz 500 ms (number of pulse at 10ms )

Zoom in at the stimulus of 0.5Hz 500 ms (frequency )

Experiment in chicken retina  Focusing image from computer display on the retina.  the space and time frequency can be controlled.  the stimulus is from the bottom

The optic setup  45 d mirror.  Lance to focus the image.  Mechanical setup.  display that radiate the image.

Movie of the experiment

The buffer components chickenrat g/1literg/molmMg/1literg/molmM NaCl KCl NaHCO GLUKOSE L-GLUTAMAT MgCl2 6H2O 1mlPHENOLROT 7.5 ph OSMOLARIT NaH2PO4(1H2O) CaCl2(2H20)

The retina preparation