1.Genes não Corinebacterineae Específicos de C. pseudotuberculosis e espécies próximas evolutivamente (Cd) Transferidos de outros clados
txid85007[Orgn] AND chromosome[Replicon Type] AND complete[PROP] AND reference[PROP] 110,052 seqs 26 genomes: Corynebacterium (7) Mycobacterium (17) Rhodococcus (1) Nocardia (1) tBLASTn CpCDS 317 NH-CpCDS tBLASTn Other txid NH-CpCDS RGMG tBLASTn UniprotKB + nr 30 CpCDS 26 distinct C. pseudotuberculosis: SMase D Serine protease precursor 280 “no hit”
YP_ putative CAAX amino terminal protease family protein COG1807 YP_ hypothetical protein ABC0212 NP_ regulatory protein (padR family) YP_ similar to superfamily I DNA and RNA helicases and helicase subunits YP_ conserved phage-associated protein YP_ conserved phage-associated protein YP_ hypothetical phage-associated protein YP_ PTS system, fructose-specific IIBC component YP_ protein of unknown function DUF181 YP_ hypothetical protein Strop_2434 YP_ Lantibiotic dehydratase domain protein YP_ hypothetical protein Strop_2432 ZP_ Secreted subtilisin-like peptidase YP_ CAAX amino terminal protease family protein NP_ nitrite reductase periplasmic cytochrome c552 YP_ putative cytochrome c nitrite reductase, small subunit NrfH XP_ viral A-type inclusion protein, putative COG gb|AAA serine proteinase precursor** YP_ hypothetical protein MUL_3536 COG ZP_ transcriptional antiterminator, BglG ZP_ nucleoside transporter YP_ similar to superfamily I DNA and RNA helicases and helicase subunits ZP_ transcriptional antiterminator, BglG ZP_ hypothetical protein ACTODO_01808 ZP_ hypothetical protein ACTODO_01815 ZP_ hypothetical protein ACTODO_01815 ZP_ hypothetical protein ACTODO_01815 ZP_ Secreted subtilisin-like peptidase ZP_ hypothetical protein XfasaDRAFT_1247 ZP_ hypothetical protein SpneT_ De onde eles vieram?
HitOccurence in other clades
Arthrobacter sp. Bacillus clausii KSM-K16 Clostridium tetani E88 Saccharopolyspora erythraea NRRL 2338 Propionibacterium acnes Exiguobacterium sp. Salinispora tropica CNB-440 Salinispora tropica CNB-441 Salinispora tropica CNB-442 Salinispora tropica CNB-443 Idiomarina baltica OS145 Bacillus cereus E33L Bdellovibrio bacteriovorus HD100 Myxococcus xanthus DK 1622 Trichomonas vaginalis G3 Enterococcus faecalis V583 Mycobacterium ulcerans Agy99 Thermosinus carboxydivorans Bacillus sp. NRRL B Saccharopolyspora erythraea NRRL 2338 Thermosinus carboxydivorans Actinomyces odontolyticus ATCC Idiomarina baltica OS145 Xylella fastidiosa Dixon Streptococcus pneumoniae Pular esse slide
2.Homologia com baixa similaridade Caso da SMase Matrizes PSSM para COG Matrizes para novos clusters Seed Linkage
(psi)tblastn PSSM 114, hits PSSM not BLOSUM tBLASTn nr 48 hits PSSM not “nr” COG No hit COG Ex: Cp-V COG0419 Cp-V COG1178 common 4 hits: Not Corinebacterineae COG annotated Tx85007 Clusters
Cp-Draft- V COG0697-Permeases of the drug/metabolite transporter (DMT) superfamilyNostoc sp. COG0697Cyanobacteria Cp-Draft- V COG0488- ATPase components of ABC transporters with duplicated ATPase domainsListeria COG0488Firmicutes Proteobacteria Cp-Draft- V COG1191- DNA-directed RNA polymerase specialized sigma subunitTreponema pallidum COG1191Spirochaetes Cp-Draft- V COG1994-Zn-dependent proteasesNostoc sp. COG1994Cyanobacteria From 280 putative exclusive Cp-CDS, four are found by search using the PSSM matrices, but are no-hits to nr, uniprot and cog: