InfraCal TOG/TPH Analyzer, Model HATR-T2 Extraction Procedure

Collect a measured amount of sample Collect a measured amount of sample

For samples that will be stored for more than a day or for samples with a high pH: Adjust the pH to less than 2 with Hydrochloric acid or Sulfuric Acid (typically 3-5 drops depending on buffers in sample)

Add Solvent Add Solvent 10 parts sample to 1 part solvent For example: For 150 ml of sample add 15 ml of solvent

Add Solvent Add Solvent 10 parts sample to 1 part solvent For example: For 150 ml of sample add 15 ml of perchloroethylene

Shake for 2 minutes Shake for 2 minutes

Open the cap on the bottle during the 2 minute shaking to relieve the pressure Open the cap on the bottle during the 2 minute shaking to relieve the pressure

Hexane is lighter than water and will float on the top of the sample Hexane is lighter than water and will float on the top of the sample

Using a syringe or pipette, take 50 microliters of solvent extract Using a syringe or pipette, take 50 microliters of solvent extract

And deposit it in the center of the sample crystal And deposit it in the center of the sample crystal

Press the run button Press the run button

14 Oil concentration displayed Oil concentration displayed

Silica Gel Extraction The difference between TPH (Total Petroleum Hydrocarbon) and TOG (Total Oil and Grease) is the polar organics are removed from the extract using silica gel. The remaining hydrocarbons are the non-polar components considered to be TPH

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel Using a 50 microliter syringe or pipette deposit on the center of the sample plate crystal Using a 50 microliter syringe or pipette deposit on the center of the sample plate crystal

Press the run button Press the run button

14 Oil concentration displayed Oil concentration displayed

For questions please contact us at: or InfraCal TOG/TPH Analyzer Extraction Procedure