Biophysics Division -1- Biophysics Division Cellular/Molecular Imaging for Bonghan System Study Kwang-Sup Soh Biomedical Physics Lab. for Korean Medicine School of Physics, Seoul National University

Biophysics Division -2- Contents I. Introduction on Bonghan System II. Co-operative Research Progress (1)Electron Microscope (2)Atomic Force Microscope (3)Nanoparticles

Biophysics Division I. Bonghan System

Biophysics Division -4- Bonghan System Organ-Surface BH duct (on the surface of organs) (on the surface of organs) Superficial BH duct (Acupuncture meridian) Superficial BH Corpuscle (Acupoints) skin : superficial BH ducts skin : superficial BH ducts network surface of organs : OSBHD network surface of organs : OSBHD inside vessels : intra-vascular BHD inside vessels : intra-vascular BHD Intravascular BH duct

Biophysics Division -5- Physiological Significance New Circulation System  Hyaluronic acid, Amino acid, Mono nucleotide,  Hormone: Adrenalin/noradrenalin Bonghan Granules (Ф≈0.8~2.4μm) containing DNA  Adult stem cells: cell therapy  Natural “micro-cell”: gene therapy

Biophysics Division II. Research Progress

Biophysics Division -7- Future Prospects AnatomyPhysiology Electron Microscopes AFMNanobiotechnology Optical Tweezer 2005 Sept, CKC Symposium

Biophysics Division -8- Electron Microscopes Microscopy Research & Technology, 2006 Electron Microscopic Study of Novel Threadlike Structures on the Surfaces of Mammalian Organs Byung-Cheon Lee, 1 Jung Sun Yoo, 1 Vyacheslav Ogay, 1 Ki Woo Kim, 2 Harald Dobberstein, 3 Kwang-Sup Soh, 1, † and Byung-Soo Chang 4, † 1 Biomedical Physics Laboratory, FPRD, School of Physics and Astronomy, Seoul National University, Seoul, Korea 2 National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea 3 Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK 4 Department of Cosmetology, Hanseo University, Seosan, Korea

Biophysics Division -9- Electron Microscopes Fig.1. Stereomicroscopic Images of novel threadlike structures

Biophysics Division -10- Electron Microscopes SEM

Biophysics Division -11- Electron Microscopes TEM

Biophysics Division -12- Electron Microscopes Cryo-SEM FIB/SEM (Cavendish)

Biophysics Division -13- Atomic Force Microscope Investigation of morphology and physical properties of Bonghan microcell using AFM and TEM 1 Ku Youn Baik, 1 Joonhyung Kwon, 1 Byung –Cheon Lee, 2 Harald Dobberstein, and 1 Kwang-Sup Soh $ 1 Biomedical Physics Laboratory, Department of Physics, Seoul National University, Seoul, , Korea 2 Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK Molecular Cell Biology GRC, NH, USA Microcell-like granules in the Bonghan corpuscle on the surfaces of mammalian internal organs 1 Ku Youn Baik, 1 Vyacheslav Ogay, 2 Harald Dobberstein, and 1 Kwang-Sup Soh 1 Biomedical Physics Laboratory, Department of Physics, Seoul National University, Seoul, , Korea 2 Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK WC2006 Conference, Seoul, Korea

Biophysics Division -14- Atomic Force Microscope Bonghan microcell cycle (Bonghan Kim, 1965) (1) All the morphological components of organism are ceaselessly reproduced. (2) The self-reproduction of organism takes the form of Bonghan microcell-cell cycles. (3) The self-reproduction of organism is performed by the acupuncture-meridian system. Theory of Bonghan Microcell

Biophysics Division -15- A Topography, B Error, C Magnified of B, D line profile 1.4 um x 1um x 350nm sized oval Bonghan microcell. It shows clearly unique surface structure of layers and portions (B & C) whose size is from 0.2um to 0.6 um in diameter. The line profile analysis shows that the common patterns on the surface are steps of about 20nm height. This pattern is thought to be revealing the stereotype of its membrane structure. AFM image of Bonghan microcell 20nm A B C D

Biophysics Division -16- Nanobiotechnology Flowing Channel of Nanoparticles