QUALITY CONTROL: INSTI Controls Preparation/SET-UP

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Presentation transcript:

QUALITY CONTROL: INSTI Controls Preparation/SET-UP For sampling of the controls with the provided single use short-stem bulb pipette, follow these instructions: Open an INSTI HIV-1/HIV-2 Antibody Test package by tearing the slit found along the side, or open a box of 24 INSTI HIV-1/HIV-2 Antibody Tests and gather one of each kit materials required. Open the individually wrapped membrane unit by tearing the slit located along the side. Make sure the membrane unit tab is facing towards you. Locate Solution 1 and twist off the red cap, set cap and opened bottle in front of you.

INSTI CONTROLS The HIV-1 Positive and Negative Controls are used to ensure that the test functions correctly. Controls are also run as part of your laboratory’s standard quality control procedures. The HIV-1 Positive Control will produce a weakly reactive result in the INSTI HIV-1/HIV-2 Antibody Test. T he HIV-2 Positive Control will produce a weakly reactive result in the INSTI HIV-1/HIV-2 Antibody Test. The HIV Negative Control will produce a non-reactive result in the INSTI HIV-1/HIV-2 Antibody Test.

USE OF INSTI CONTROLS: STEP 1 Use a new pipette for each Control sample collection. Take the pipette and lightly depress the top bulb.

USE OF INSTI CONTROLS: STEP 2 Insert pipette tip into the clear liquid in the control vial. Slowly release the top bulb to completely fill the pipette stem.

USE OF INSTI CONTROLS: STEP 3 Ensure that the liquid in the stem reaches ONLY to the fill line as indicated by the arrow in the photograph. Keep the bulb slightly depressed to retain the liquid in the stem. Do not allow the liquid to be drawn into the bulb.

USE OF INSTI CONTROLS: STEP 4 Take the pipette and lightly depress the top bulb to express the contents into the Solution 1 vial. Use a new pipette for each Control sample collection.

INSTI CONTROLS Do’s and Don’ts Do: read and become familiar with the INSTI Controls package insert. Do: pipette carefully to avoid over- or under sampling. Each control vial should have enough volume for 8 INSTI tests. Do Not: return control vials to the freezer once they have been opened. Store at 2-8C for up to 28 days. Do Not: reuse the bulb pipettes. They are single use, disposable “consumables”.