Fig. 3 Single-molecule DNA recovery and amplification.

Slides:

Advertisements

Similar presentations

Fig. 5 Correlation of RNA expression and protein abundance.

Advertisements

Fig. 2 Transport properties of a BP transistor at low temperature.

Fig. 4 Ballistic simulation of BP FETs.

Fig. 3 Cytosolic delivery of fluorescent proteins.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)

Fig. 1 Typical presbyopic vision with various methods of correction.

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 1 Map of water stress and shale plays.

Fig. 2 Self-assembly of NPs in the Ch-CNC droplets.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 1 NP-free Ch-CNC droplets.

Fig. 1 Wireless, battery-free neural cuff for programmable pharmacology and optogenetics. Wireless, battery-free neural cuff for programmable pharmacology.

Fig. 3 Electron PSD in various regions.

Fig. 4 Morphogenesis in the Ch-CNC host droplets and NP assemblies.

Fig. 6 Different models of assembly of Ch-CNC droplets laden with Fe3O4NPs under magnetic field. Different models of assembly of Ch-CNC droplets laden.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

HT synthesis of boronic acids using the building block approach

Fig. 6 Comparison of properties of water models.

Fig. 2 Reference-fixing experiment, results.

Fig. 3 Scan rate effects on the layer edge current.

Fig. 1 Experimental apparatus used to train and test free-flying bees on their capacity to learn addition and subtraction. Experimental apparatus used.

Interactive morphogenesis in Ch-CNC droplets laden with magnetic NPs

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Reference-fixing experiment, setup.

Fig. 1 Schematic illustration of the preparation and potential application of FMSMs. Schematic illustration of the preparation and potential application.

Fig. 1 Distribution of total and fake news shares.

Fig. 1 Schematic illustrating the combined use of bioorthogonal ligation (i.e., the reaction between Tz and TCO) and disulfide cleavage–driven by DTT to.

Fig. 3 Characteristics of UV and temperature sensors.

Fig. 2 2D QWs of different propagation lengths.

Fig. 1 Structure of L10-IrMn.

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 3 Proteomic analysis and Western blot analysis of protein cargos of various EVs. Proteomic analysis and Western blot analysis of protein cargos of.

Fig. 4 DFT ωB97x/def2-TZVPP atomic charges on the sulfur atom of substituted thioaldehyde and AIMNet prediction with a different number of iterative passes.

Fig. 5 In-plane angle dependence of SOT efficiency (θDL,m) and resonance condition (Hres). In-plane angle dependence of SOT efficiency (θDL,m) and resonance.

Fig. 1 Histograms of the number of first messages received by men and women in each of our four cities. Histograms of the number of first messages received.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

Fig. 5 Topology structure and strategy for the bottom-up synthesis of structurally uniform carboncones[1,m]. Topology structure and strategy for the bottom-up.

Fig. 1 Average contribution (million metric tons) of seafood-producing sectors, 2009–2014. Average contribution (million metric tons) of seafood-producing.

Fig. 4 Praying Prophet by Lorenzo Monaco: Mapping lake pigments and associated substrate. Praying Prophet by Lorenzo Monaco: Mapping lake pigments and.

Fig. 1 Cross-sectional images of He-implanted V/Cu/V samples.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

Fig. 2 Schematic drawings of Göbekli Tepe skulls.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 2 Top 10 countries, ecoregions, conservation hotspots, and KBAs with the largest area of restoration hotspots. Top 10 countries, ecoregions, conservation.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Schematic of the proposed brain-controlled assistive hearing device

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 2 6-HAP does not inhibit DNA synthesis in vitro.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 3 Directional rolling of an NP on a dsDNA fragment with flexibility gradient. Directional rolling of an NP on a dsDNA fragment with flexibility gradient.

Fig. 2 Simulations of possible doping positions and band structures.

Fig. 2 Mean field results. Mean field results. (A) Solutions P(x) to Eq. 4 for a range of T and wc = (B) Modulus ∣pk∣ of order parameters versus.

Fig. 4 The relationship between the total mean absolute momentum disturbance 〈∣p∣〉zB (in units of ℏ/D) and fringe visibility V. The relationship between.

Fig. 1 Schematic depiction of a paradigm for rapid and guided discovery of materials through iterative combination of ML with HiTp experimentation. Schematic.

Fig. 4 No genetic divergence of HIV during cART.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 4 Single-particle contact angle measurements.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 4 Behavior of resistance peak near density nm = 5.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Fig. 1 B2 RNA directly inhibits transcription.

Fig. 1 Architected materials fabrication by projection microstereolithography–based additive manufacturing using poly(ethylene glycol) diacrylate resin.

Fig. 1 Doping schematics and optical properties.

Fig. 4 Effects of individual picosecond and microsecond pulses.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 1 Completely derived from natural wood, nanowood with hierarchically aligned cellulose nanofibrils can be used as an anisotropic super thermal insulator.

Fig. 2 Proportion of time the listener spent looking upward across original recording conditions of the playback stimuli. Proportion of time the listener.

Presentation transcript:

Fig. 3 Single-molecule DNA recovery and amplification. Single-molecule DNA recovery and amplification. (A) Schematic workflow for the recovery of single DNA molecules. The silicone rubber [polydimethylsiloxane (PDMS)] can be peeled off from the array substrate before recovery. The recovery is carried out with a glass microcapillary equipped on an XYZ-axis manipulator. Every droplet of interest is determined via digital image processing, which allows for fast addressing and moving of the microcapillary toward target positions. Each recovered sample is subjected to amplification [polymerase chain reaction (PCR) or reverse transcription PCR (RT-PCR)], making conventional detection and purification of nucleic acids available. The purified DNA can directly be used for various kinds of downstream applications. (B) Proof of demonstration of nucleic acid recovery using fluorescent proteins. After mVenus synthesis, individually addressable droplets allowed a microcapillary prefilled with water to suck up whole contents out of the microchamber without disturbing surrounding droplets. Each recovered mRNA (lanes 1 to 4) or DNA (lanes 5 to 8) can all be amplified efficiently with RT-PCR followed by another round of PCR, or two rounds of PCR, respectively. The process featuring the changes of bright field and fluorescence before and after the recovery was recorded in movie S5. NC, negative control (nonfluorescent droplet) for DNA amplification; PC, positive control (purified target DNA, 1010 bp); M1, 100-bp DNA ladder; M2, 500-bp DNA ladder. Yi Zhang et al. Sci Adv 2019;5:eaav8185 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).