Test of equal amplification efficiency of competitive PCR by titration of competitor. Test of equal amplification efficiency of competitive PCR by titration.

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Test of equal amplification efficiency of competitive PCR by titration of competitor. Test of equal amplification efficiency of competitive PCR by titration of competitor. (A) PAGE of IL-2 competitive RT-PCR of changing input ratio series with increasing amount of competitor left to right. (B)–(D) The output native/competitor ratios of each series is plotted against input ratios relative to the one or two output ratios closest to 1. The actual data lie close to the line of prediction where output ratios equal input ratios. The line of prediction is indicated by a dashed line with a slope of 1. (B) Duplicate IL-2 RT-PCR were conducted for 35 and 40 cycles. β-actin (C) and GAPDH (D) titrations were replicated (opened and closed objects) at 40 cycles. E M Glare et al. Thorax 2002;57:765-770 Copyright © BMJ Publishing Group Ltd & British Thoracic Society. All rights reserved.