Fig. 2 Functional validation of the TaWAK gene by mutagenesis and transgenesis. Functional validation of the TaWAK gene by mutagenesis and transgenesis.

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Fig. 2 Functional validation of the TaWAK gene by mutagenesis and transgenesis. Functional validation of the TaWAK gene by mutagenesis and transgenesis. (A) Gene structure of the TaWAK (Snn1) gene with exons in yellow and UTRs in gray. Red arrowheads indicate mutation sites in EMS-induced mutants, all of which were insensitive to SnTox1. PKC, protein kinase C. (B) Infiltration and inoculation reactions on leaves of wild-type CS and the EMS mutant CSems6125 are shown as an example. (C) Reactions to SnTox1 infiltrations of CS (Snn1+), untransformed Bobwhite (BW; Snn1−), and sensitive and insensitive T1 transgenic plants both derived from the same event (BW5240). (D) Transgenic plants that were sensitive to SnTox1 were also susceptible to disease caused by spores of an SnTox1-producing fungal isolate. (E) All SnTox1-sensitive T1 plants derived from event BW5240 had the TaWAK transgene, and all insensitive plants lacked the transgene. (F) Similarly, all SnTox1-sensitive BW5240 T1 plants expressed TaWAK, whereas the insensitive plants did not. Gongjun Shi et al. Sci Adv 2016;2:e1600822 Copyright © 2016, The Authors