Osmotic Stress Affects S6K1 Kinase Activity. (A) Leaves of tobacco (N

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Osmotic Stress Affects S6K1 Kinase Activity. (A) Leaves of tobacco (N Osmotic Stress Affects S6K1 Kinase Activity.(A) Leaves of tobacco (N. benthamiana) transiently expressing 35S:GFP-S6K1 construct were treated with different concentration of mannitol. Osmotic Stress Affects S6K1 Kinase Activity.(A) Leaves of tobacco (N. benthamiana) transiently expressing 35S:GFP-S6K1 construct were treated with different concentration of mannitol. GFP-S6K1 protein was then immunoprecipitated with GFP antibody and subjected to in vitro kinase assay using GST-RPS6-CT as substrate. Top panel: protein gel blot showing equal loading of S6K1 in the kinase reaction. Bottom panel: autoradiograph showing the activity of S6K1 under different concentrations of mannitol treatment.(B) Quantification of the kinase activity shown in (A) after normalization with the intensity of S6K1 bands in the protein gel blot. The results are represented as the average of four independent experiments ± se (n = 4, P < 0.01 by analysis of variance). Magdy M. Mahfouz et al. Plant Cell 2006;18:477-490 ©2006 by American Society of Plant Biologists