Decreased enzymatic activity in PKG mutants increases time to recovery from an electroshock. Decreased enzymatic activity in PKG mutants increases time.

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Decreased enzymatic activity in PKG mutants increases time to recovery from an electroshock. Decreased enzymatic activity in PKG mutants increases time to recovery from an electroshock.Drosophila melanogaster third-instar larvae (n≥30) were stimulated at 12 V in the larval electroconvulsive seizure assay. Sitter larvae took less time to recover from a seizure when compared with the foraging null mutant (for0, no PKG) whereas the recovery time of the null rescue (forrescue) and overexpressor (foroverex) lines, which express low levels of PKG, was not significantly different from that of the sitter larvae. Bars represent means±s.e.m. and asterisks denote a significant difference (***P≤0.001). Significance was determined using a one-way ANOVA on ranks followed by Tukey's post hoc multiple comparisons test. Stephanie P. Kelly et al. J Exp Biol 2018;221:jeb179747 © 2018. Published by The Company of Biologists Ltd