Volume 54, Pages S159-S161 (September 1998)

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Volume 54, Pages S159-S161 (September 1998) Detection of multiple vascular endothelial growth factor splice isoforms in single glomerular podocytes Matthias Kretzler, Bernd Schröppel, Monika Merkle, Stephan Huber, Peter Mundel, Michael Horster, Detlef Schlöndorff Kidney International Volume 54, Pages S159-S161 (September 1998) DOI: 10.1046/j.1523-1755.1998.06733.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Photomicrograph demonstrating the podocyte aspiration technique: fixed glomerular tuft (left), single podocyte cell soma aspirated in the tip of a micropipette (right). Kidney International 1998 54, S159-S161DOI: (10.1046/j.1523-1755.1998.06733.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 (A) VEGF expression determined by reverse transcriptase-polymerase chain reaction (RT-PCR) representative reactions. From left to right: mRNA from whole cortex mRNA (Renal Cortex), from isolated single glomerulus (Isolated Glom), podocyte cell culture (Cultured podocytes), un-diff.: undifferentiated cobblestone phenotype, diff.: differentiated arborized phenotype, isolated single podocyte: Single podocyte. Vascular endothelial growth factor (VEGF) isoforms are indicated by arrows. Note the up-regulation of VEGF in differentiated podocytes. (B) Gene structure of the different VEGF splice isoforms. Arrowheads mark position of sense and antisense RT-PCR primer1. Kidney International 1998 54, S159-S161DOI: (10.1046/j.1523-1755.1998.06733.x) Copyright © 1998 International Society of Nephrology Terms and Conditions