The Level of Prosaposin is Decreased in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Silke Stachowitz, Johannes Ring, Heidrun.

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Presentation transcript:

The Level of Prosaposin is Decreased in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Silke Stachowitz, Johannes Ring, Heidrun Behrendt Journal of Investigative Dermatology Volume 116, Issue 3, Pages 394-400 (March 2001) DOI: 10.1046/j.1523-1747.2001.01283.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Gelelectrophoresis of pSAP RT-PCR products. Gelelectrophoresis of GAPDH (upper lanes) and pSAP (lower lanes) in normal control skin (C), and in three psoriatic patients (P1, P2, P3, uninvolved skin (a), and involved skin (b)). Total cellular RNA was isolated from epidermal specimen, and after PCR, reaction products were visualized after electrophoresis of ethidium bromide-stained agarose gel as described (see Materials and Methods). Journal of Investigative Dermatology 2001 116, 394-400DOI: (10.1046/j.1523-1747.2001.01283.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Semiquantitative analysis of pSAP PCR bands. Relative density levels of pSAP PCR bands are expressed in percentage of GAPDH. C = Normal control skin; P1, P2, P3 = three psoriatic patients, uninvolved skin left, involved skin right. Densitometric values obtained from the corresponding ethidium bromide-stained PCR reaction products in Figure 1 are presented. Journal of Investigative Dermatology 2001 116, 394-400DOI: (10.1046/j.1523-1747.2001.01283.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Immunohistochemical localization of pSAP-like protein and nonprimary negative control in normal control skin. (a) pSAP-like protein; (b) nonprimary negative control. The immunohistochemical study was performed using the ABC method (see Materials and Methods). pSAP-like protein was localized as dotty reaction in all epidermal layers, but especially in the granular-corneocyte transition zone and in the stratum corneum (a, arrow). No stain was detected using a nonspecific primary antibody (b). Scale bar: 30 μm. Journal of Investigative Dermatology 2001 116, 394-400DOI: (10.1046/j.1523-1747.2001.01283.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Immunohistochemical localization of pSAP-like protein in psoriatic epidermis. In nonlesional psoriatic epidermis (a) pSAP-like protein is visible in the granular-corneocyte transition zone and in the lower stratum corneum (arrows). In lesional psoriatic epidermis (b) pSAP-like protein is decreased in whole epidermis, but especially in the stratum corneum. Spotty reaction are confined to the granular-corneocyte transition zone (arrow). Positive staining of an inflammatory cell in the dermis is also evident (small arrow). Scale bar: 30 μm. Journal of Investigative Dermatology 2001 116, 394-400DOI: (10.1046/j.1523-1747.2001.01283.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Immunohistochemical localization of SMase in psoriatic epidermis. SMase was immunohistochemically detected in tissue sections of nonlesional and lesional psoriatic skin using the ABC method (see Materials and Methods). In nonlesional psoriatic skin (a) SMase can be visualized in the periphery of and weakly within the cells of stratum spinosum, stratum granulosum, lucidum, and stratum corneum (arrow), being absent in the stratum basale (small arrow). In lesional psoriatic epidermis (b) SMase is localized as dotty reactions in the granular-corneocyte transition zone (arrow). Scale bar: 30 μm. Journal of Investigative Dermatology 2001 116, 394-400DOI: (10.1046/j.1523-1747.2001.01283.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions