Platelet cytoplasmic calcium concentration in PI3Kγ-null platelets.

Slides:

Advertisements

Similar presentations

Red blood cells mediate the onset of thrombosis in the ferric chloride murine model by Justin D. Barr, Anil K. Chauhan, Gilbert V. Schaeffer, Jessica K.

Advertisements

Human Keratinocytes Express Multiple P2Y-Receptors: Evidence for Functional P2Y1, P2Y2, and P2Y4 Receptors Helen E. Burrell, Wayne B. Bowler, James A.

Acalabrutinib and ibrutinib therapy cause dysfunctional GPVI-mediated platelet aggregation. Acalabrutinib and ibrutinib therapy cause dysfunctional GPVI-mediated.

Regulation of platelet plug formation by phosphoinositide metabolism

Reduced thrombus stability in mice lacking the α2A-adrenergic receptor

Activation of αIIbβ3 is a sufficient but also an imperative prerequisite for activation of α2β1 on platelets by Gerlinde R. Van de Walle, Anne Schoolmeester,

by Matt W. Goschnick, Lai-Man Lau, Janet L. Wee, Yong S. Liu, P

Tissue-Specific Expression of Functional Platelet Factor XI Is Independent of Plasma Factor XI Expression by Chang-jun Hu, Frank A. Baglia, David C.B.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

Vessel wall BAMBI contributes to hemostasis and thrombus stability

Regulation of FcεRI-mediated degranulation by an adaptor protein 3BP2 in rat basophilic leukemia RBL-2H3 cells by Kiyonao Sada, S. M. Shahjahan Miah, Koichiro.

by Kesheng Dai, Richard Bodnar, Michael C. Berndt, and Xiaoping Du

Essential role for phosphoinositide 3-kinase in shear-dependent signaling between platelet glycoprotein Ib/V/IX and integrin αIIbβ3 by Cindy L. Yap, Karen.

Recombinant factor VIIa restores aggregation of αIIbβ3-deficient platelets via tissue factor–independent fibrin generation by Ton Lisman, Jelle Adelmeijer,

Effects of inflammatory cytokines on the release and cleavage of the endothelial cell–derived ultralarge von Willebrand factor multimers under flow by.

Rituximab-induced tumor cell agglutination

Intracellular labile iron pools as direct targets of iron chelators: a fluorescence study of chelator action in living cells by Hava Glickstein, Rinat.

Necrotic platelets provide a procoagulant surface during thrombosis

by Nayna Mistry, Susan L. Cranmer, Yuping Yuan, Pierre Mangin, Sacha M

by Gail Dallalio, Erin Law, and Robert T. Means

by Zhengyan Wang, Tina M. Leisner, and Leslie V. Parise

by Daniel W. Bougie, Ana I. Benito, Luis I

Conjugation of Blocked Ricin to an Anti-CD19 Monoclonal Antibody Increases Antibody-Induced Cell Calcium Mobilization and CD19 Internalization by Anne-Christine.

Inhibition of collagen-induced platelet aggregation by anopheline antiplatelet protein, a saliva protein from a malaria vector mosquito by Shigeto Yoshida,

Elevated prothrombin results in clots with an altered fiber structure: a possible mechanism of the increased thrombotic risk by Alisa S. Wolberg, Dougald.

by Laurent O. Mosnier, Paula Buijtenhuijs, Pauline F. Marx, Joost C. M

A role for the thiol isomerase protein ERP5 in platelet function

A potential role for α-actinin in inside-out αIIbβ3 signaling

Macrophages from C3-deficient mice have impaired potency to stimulate alloreactive T cells by Wuding Zhou, Hetal Patel, Ke Li, Qi Peng, Marie-Bernadette.

Rap1-GTP–interacting adaptor molecule (RIAM) is dispensable for platelet integrin activation and function in mice by Simon Stritt, Karen Wolf, Viola Lorenz,

Β1 integrin−mediated signals are required for platelet granule secretion and hemostasis in mouse by Tobias Petzold, Raphael Ruppert, Dharmendra Pandey,

Impaired activation of platelets lacking protein kinase C-θ isoform

by Robert McMillan, Jennifer Lopez-Dee, and Joseph C. Loftus

Oxidized low-density lipoproteins induce rapid platelet activation and shape change through tyrosine kinase and Rho kinase–signaling pathways by Katie.

Phosphorylation of CLEC-2 is dependent on lipid rafts, actin polymerization, secondary mediators, and Rac by Alice Y. Pollitt, Beata Grygielska, Bertrand.

Vascular endothelial growth factor stimulates protein kinase CβII expression in chronic lymphocytic leukemia cells by Simon T. Abrams, Benjamin R. B. Brown,

IRAG mediates NO/cGMP-dependent inhibition of platelet aggregation and thrombus formation by Melanie Antl, Marie-Luise von Brühl, Christina Eiglsperger,

by A. C. Azim, K. Barkalow, J. Chou, and J. H. Hartwig

Rapid ubiquitination of Syk following GPVI activation in platelets

Α-Melanocyte-Stimulating Hormone, MSH 11–13 KPV and Adrenocorticotropic Hormone Signalling in Human Keratinocyte Cells Richard J. Elliott, Marika Szabo,

Ubiquitination and degradation of the thrombopoietin receptor c-Mpl

RGS10 shapes the hemostatic response to injury through its differential effects on intracellular signaling by platelet agonists by Peisong Ma, Shuchi Gupta,

In-depth PtdIns(3,4,5)P3 signalosome analysis identifies DAPP1 as a negative regulator of GPVI-driven platelet function by Tom N. Durrant, James L. Hutchinson,

Enhancing functional platelet release in vivo from in vitro–grown megakaryocytes using small molecule inhibitors by Danuta Jarocha, Karen K. Vo, Randolph.

Cytotoxic activities of polymer micelles containing CFZ in H460 (A) and select micelle formulations in RPMI-8226 (B) cell lines. Cytotoxic activities of.

Cytotoxic effects of PM1 and PM2 empty particle controls and coincubation of CFZ with empty particles tested in H460 (A) and RPMI-8226 (B) cells. Cytotoxic.

Thrombospondin-1 stimulates platelet aggregation by blocking the antithrombotic activity of nitric oxide/cGMP signaling by Jeff S. Isenberg, Martin J.

Rap1 binding to the talin 1 F0 domain makes a minimal contribution to murine platelet GPIIb-IIIa activation by Frederic Lagarrigue, Alexandre R. Gingras,

TNF-α–stimulated adhesion of HL60 cells to HCMEC is inhibited by neuraminidase but not by NaClO3 . TNF-α–stimulated adhesion of HL60 cells to HCMEC is.

Severe platelet dysfunction in NHL patients receiving ibrutinib is absent in patients receiving acalabrutinib by Alexander P. Bye, Amanda J. Unsworth,

by Ludovic Durrieu, Alamelu Bharadwaj, and David M. Waisman

Soluble GPVI is elevated in injured patients: shedding is mediated by fibrin activation of GPVI by Samantha J. Montague, Céline Delierneux, Christelle.

Intracellular calcium response of endothelial cells exposed to flow in the presence of thrombin or histamine Laura M. Worthen, MS, Matthias U. Nollert,

by Caroline Kardeby, Knut Fälker, Elizabeth J

Predicted number of joint bleeds according to factor activity level and age group for patients with hemophilia A or B based on a regression model. Predicted.

Calpain cleaves phospholipid flippase ATP8A1 during apoptosis in platelets by Weidong Jing, Mehmet Yabas, Angelika Bröer, Lucy Coupland, Elizabeth E. Gardiner,

Secretion is ablated in the absence of SNAP23.

Platelet cytoplasmic calcium concentration in PLCβ2/β3-null platelets.

Platelet spreading on immobilized fibrinogen.

Platelet ATP secretion in response to agonist stimulation.

Immunoblots of PLCβ isoforms in wild-type and PLCβ2/β3-null platelets.

Aggregation tracings of platelets lacking PLCβ2/β3.

Actin assembly in response to thrombin.

Involvement of IRS-1 in L-783,281–stimulated increases in [Ca2+]i and exocytosis. Involvement of IRS-1 in L-783,281–stimulated increases in [Ca2+]i and.

Synthetic glycopolymers require GPIbα to induce full aggregation in murine and human platelets. Synthetic glycopolymers require GPIbα to induce full aggregation.

Electron microscopic view shows one tumor cell with a round regular nucleus (N), peripherally clumped chromatin (short arrows), and surrounding small amount.

Src kinases mediate TSP-1 inhibition of AC and PKA activity.

by John C. Kermode, Qi Zheng, and Elizabeth P. Milner

Distinct mitochondrial membrane potential, ATP-to-ADP ratio and NAD(P)H responses to glucose and methylsuccinate. Distinct mitochondrial membrane potential,

A binding relationship with thrombin

Presentation transcript:

Platelet cytoplasmic calcium concentration in PI3Kγ-null platelets. Platelet cytoplasmic calcium concentration in PI3Kγ-null platelets. Washed murine platelets were incubated with a calcium fluorophore (Fura-2 AM) and then stimulated with either 1 U/mL thrombin or 10 mM ADP. The concentration of cytoplasmic calcium (nM) was quantitated as a function of time. (A) Representative fluorimetry tracings of experiments using thrombin- or ADP-stimulated wild-type and PI3Kγ-null platelets. (B) A graph displaying the mean ± SEM of the cytosolic calcium concentration for 3 experiments. (C) A graph demonstrating the influence of the extracellular calcium concentration on the ability of platelets to raise their cytoplasmic calcium concentration in response to 1 U/mL thrombin. The change in cytosolic calcium was normalized to the response seen in wild-type cells. The graph shows the mean ± SEM for 3 experiments. Lurong Lian et al. Blood 2005;106:110-117 ©2005 by American Society of Hematology