Roche NimbleGen oligonucleotide microarray.

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Presentation transcript:

Roche NimbleGen oligonucleotide microarray. Roche NimbleGen oligonucleotide microarray. Maskless array synthesizer technology is depicted, which utilizes a digital micromirror device (DMD) to create virtual masks. The DMD forms the pattern of UV light needed to direct the specific nucleic acid addition during photo-mediated synthesis. UV light removes the photolabile protecting group (circles) from the microarray surface, allowing the addition of a single protected nucleotide to the growing oligonucleotide chain. The cycling of DMD filtering, light deprotection, and nucleotide addition creates oligonucleotide features 60 to 100 bp in length on the NimbleGen microarray. (Courtesy of Roche NimbleGen [copyright Roche NimbleGen, Inc.].)‏ Melissa B. Miller, and Yi-Wei Tang Clin. Microbiol. Rev. 2009; doi:10.1128/CMR.00019-09