Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus

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Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus P. Basset, L. Senn, G. Prod’hom, J. Bille, P. Francioli, G. Zanetti, D.S. Blanc Clinical Microbiology and Infection Volume 16, Issue 8, Pages 1289-1296 (August 2010) DOI: 10.1111/j.1469-0691.2009.03070.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Double locus sequence typing (DLST) single locus variant (SLV) clustering using eBurst on the 1242 MRSA isolates recovered in the Vaud canton (Switzerland) between 2005 and 2007. Each circle represents one DLST-type and the diameter of the circles reflects the frequency of that type (i.e. the number of isolates). Linked DLST-type differ at one of the two loci (clfB or spa). For each cluster, the founder and sub-founder are indicated in blue and yellow, respectively. DLST and staphylococcal cassette chromosome mec (SCCmec) types of each founder and sub-founder are indicated in bold. For each SLV, SCCmec is only indicated (in green) when it differs from the founder. Unidentified SCCmec types are indicated by the letter N. DLST types carrying PVL-positive isolates are indicated by red plus (+) signs. Signs in parentheses indicate that not all the strains of that DLST-type have this characteristic. Clinical Microbiology and Infection 2010 16, 1289-1296DOI: (10.1111/j.1469-0691.2009.03070.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Similarities of pulsed field gel electrophoresis (PFGE) restriction patterns of some representative strains of the Swiss clone C. Double locus sequence typing (DLST) type, spa type, sequence type (ST), clonal complex (CC), staphylococcal cassette chromosome mec (SCCmec) type, and presence of PVL genes were also indicated when available. Similarities between strains were calculated with the Dice coefficient and a band position tolerance of 1.5% (defined as the lowest tolerance that enable 100% similarity between patterns of a control strain included in each gel). The dendrogram was constructed using the unweighted pair group method with arithmetic mean algorithm. Clinical Microbiology and Infection 2010 16, 1289-1296DOI: (10.1111/j.1469-0691.2009.03070.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions