Figure 4. RLS spectra of (A) TMPipEOPP and (B) OMHEPzEOPP in the presence of different concentrations of KRAS. The RLS ... Figure 4. RLS spectra of (A)

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J Exp Bot. 2017;68(17): doi: /jxb/erx352

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Figure 4. RLS spectra of (A) TMPipEOPP and (B) OMHEPzEOPP in the presence of different concentrations of KRAS. The RLS ... Figure 4. RLS spectra of (A) TMPipEOPP and (B) OMHEPzEOPP in the presence of different concentrations of KRAS. The RLS intensity increased first (red lines) and then declined (blue lines) with KRAS concentration. The inserts show the KRAS concentration-dependent changes in the RLS signal intensity at 450 nm. [TMPipEOPP] = [OMHEPzEOPP] = 5 μM. Excitation and emission slits were set at 3 and 1.5 nm, respectively. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Scheme 1. The chemical structures of the four porphyrin derivatives used in this work. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Scheme 2. Synthetic route of OMHEPzEOPP. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 1. (A, B) UV-vis absorption, (C, D) fluorescent, (E, F) RLS spectra and corresponding signal outputs of ... Figure 1. (A, B) UV-vis absorption, (C, D) fluorescent, (E, F) RLS spectra and corresponding signal outputs of TMPipEOPP in the absence or presence of different DNAs at pH of 7.0. Free porphyrin (black line); ssDNA (green line); dsDNA (blue line); G-quadruplex (red line). The effects of other DNAs were shown in Supplementary Figures S8–S11. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. RLS intensity ratio of (A) OMHEPzEOPP, (B) TMPipEOPP, (C) m-TPyEOPP and (D) FA-TMPipEOPP in the absence or ... Figure 2. RLS intensity ratio of (A) OMHEPzEOPP, (B) TMPipEOPP, (C) m-TPyEOPP and (D) FA-TMPipEOPP in the absence or presence of different structural DNAs at pH 7.4, 7.0, 6.5 and 6.0, respectively. ‘0’ represent free porphyrins, and the serial number of DNA is same to that in Table 1. Free porphyrin (black); ssDNAs (green); dsDNAs (blue); G-quadruplexes (red). [porphyrin] = 5 μM; [DNA] = 10 μM. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 3. (A–C) DNA concentration-dependent RLS spectral changes of TMPipEOPP. (A) KRAS (G-quadruplex); (B) CtDNA ... Figure 3. (A–C) DNA concentration-dependent RLS spectral changes of TMPipEOPP. (A) KRAS (G-quadruplex); (B) CtDNA (dsDNA); (C) ssDNA1 (ssDNA). (D–G) DNA concentration-dependent RLS intensity ratio changes of the four porphyrin probes under their individual appropriate pH conditions. [porphyrin] = 5 μM. Excitation and emission slits were both set at 3 nm. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 5. The result of LDA for (A) OMHEPzEOPP, (B) TMPipEOPP, (C) m-TPyEOPP and (D) FA-TMPipEOPP in the absence or ... Figure 5. The result of LDA for (A) OMHEPzEOPP, (B) TMPipEOPP, (C) m-TPyEOPP and (D) FA-TMPipEOPP in the absence or presence of different structural DNAs at pH 7.4, 7.0, 6.5 and 6.0, respectively. ssDNA and dsDNA (yellow); G-quadruplex (purple). Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 6. Let-7a detection using TMPipEOPP as RLS probe Figure 6. Let-7a detection using TMPipEOPP as RLS probe. (A) Working mechanism of the sensing platform. (B) RLS spectra ... Figure 6. Let-7a detection using TMPipEOPP as RLS probe. (A) Working mechanism of the sensing platform. (B) RLS spectra of the sensing solutions in the presence of different concentrations of Let-7a. (C) Let-7a concentration-dependent changes in RLS intensity ratio of I<sub>702</sub>/I<sub>652</sub>. The insert shows the linear relationship between I<sub>702</sub>/I<sub>652</sub> value and Let-7a concentration in the range of 0–3.0 μM. [TMPipEOPP] = 5 μM. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, Volume 47, Issue 6, 04 February 2019, Pages 2727–2738, https://doi.org/10.1093/nar/gkz064 The content of this slide may be subject to copyright: please see the slide notes for details.