Top-down analysis of intact bovine carbonic anhydrase II by LTQ Orbitrap Velos. Top-down analysis of intact bovine carbonic anhydrase II by LTQ Orbitrap.

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.

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Top-down analysis of intact bovine carbonic anhydrase II by LTQ Orbitrap Velos. Top-down analysis of intact bovine carbonic anhydrase II by LTQ Orbitrap Velos. A, Orbitrap MS full scan of (M + 24 H)24+ acquired at full isotopic resolution. Inset, deconvolution of base peak. Note that the mass error is less than one p.p.m. with external calibration. Red dotted line indicates the in silico simulation of carbonic anhydrase (M + 24 H)24+ at resolving power of 72,000. B, HCD of [M + 34 H]34+ of carbonic anhydrase. The inset shows the cleavage coverage of the protein sequence by HCD. Jesper V. Olsen et al. Mol Cell Proteomics 2009;8:2759-2769 © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.